汎用品年度末キャンペーン実施中 ~ 2018年3月31日まで
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情報全件数:64件
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分子遺伝学 > DNA組換え
製品名
 
製品簡略説明
商品コード:70-007
Rad51タンパク質は大腸菌RecAタンパク質のホモログで、酵母からヒトまで機能的、構造的に保存され、遺伝的組換え及び組換え修復において最も重要なDNA鎖の交換反応を触媒する機能をもつ。Rad51はゲノム安定性の維持一般にも重要な機能を果たし、組換え反応に関与する多くのタンパク質(Rad52 Dmc1他)や癌関連タンパク質(BRACA1 2 p53 他)などと複合体を形成する(1、2).
組換え体ヒトRad51精製タンパク質をニワトリに免疫し、卵黄から高度に(~98%) IgY(哺乳類のIgGに相当)画分を精製した。上記のIgY 10 mgを3.5 mlのcyanogen bromideで活性化した※SepharoseTM に結合させた。本品 (~100 ul)は、約280 ugのIgYを結合している。
用途 1.免疫沈降法 (native及びdenatured form共に可能)  
2.免疫共沈降によってRad51タンパク質と結合するタンパク質を同定する。 
3.抗体カラムとして用いてRad51タンパク質と結合するタンパク質を細胞抽出液から濃縮、精製する。
 
 
商品コード
容量
価格
 
70-007
100 ul
30,000円(税抜)

製品名
 
製品簡略説明
商品コード:62-025

Taf14 functions as a component of the DNA-binding general transcription factor complex TFIID the RNA polymerase II associated general transcription factor complex TFIIF and the chromatin-remodeling complex SWI/SNF. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction core promoter recognition and selectivity TFIIA and TFIIB interaction chromatin modification (histone acetylation by TAF1) facilitation of DNA opening and initiation of transcription. TFIIF is essential for the initiation of transcription by RNA polymerase II. TFIIF functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex decreasing the affinity of RNA polymerase II for non-specific DNA allowing for the subsequent recruitment of TFIIE and TFIIH and facilitating RNA polymerase II elongation. The TAF14 subunit has stimulatory activity. Component of the SWI/SNF complex an ATP-dependent chromatin-remodeling complex is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome leading eventually to a change in nucleosome position thus facilitating or repressing binding of gene-specific transcription factors. Component of the histone acetyltransferase NuA3 complex that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3. In conjunction with the FACT complex NuA3 may be involved in transcriptional regulation.
Taf14 consists of 244 amino acids with molecular mass of 27440 Da
 
 
商品コード
容量
価格
 
62-025
100 ul
39,000円(税抜)

製品名
 
製品簡略説明
商品コード:62-026

Tfa1 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase
Taf14 consists of 482 amino acids with molecular mass of 54742 Da
 
 
商品コード
容量
価格
 
62-026
100 ul
39,000円(税抜)

製品名
 
製品簡略説明
商品コード:62-027

Ada2 functions as component of the transcription regulatory histoneacetylation (HAT) complexes SAGA SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 SPT8 and SPT20) and promoter selectivity interaction with transcription activators (GCN5 ADA2 ADA3 and TRA1) and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac H3K14ac H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA an altered form of SAGA may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3 at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B
Ada2 consists of 434 amino acids with molecular mass of 50569 Da
 
 
商品コード
容量
価格
 
62-027
100 ul
39,000円(税抜)

製品名
 
製品簡略説明
商品コード:62-028

Ngg1 transcription regulator. Could inhibit GAL4 DNA-binding or its ability to activate transcription. Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA SALSA SLIK and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 SPT8 and SPT20) and promoter selectivity interaction with transcription activators (GCN5 ADA2 ADA3 and TRA1) and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac H3K14ac H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA an altered form of SAGA may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3 at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (at Lys-14 and Lys-18) and H2B.
NGG1 consists of 702 amino acids with molecular mass of 79282 Da
 
 
商品コード
容量
価格
 
62-028
100 ul
39,000円(税抜)

分子遺伝学 > DNA組換え
製品名
 
製品簡略説明
62-101

保存: 4℃で送付、-20℃で保存

反応性:出芽酵母Rad51タンパク質. 反応特異性はrad51 変異株のWBによって確認(図1)

免疫原:His-TagをN-末につけた全長の S.c Rad51組換え体タンパク質(大腸菌で発現)

用途
1)ウェスタンブロッテイング(2000~10000倍希釈)(図12)。
2)免疫沈降実験によって、種々のタンパク質との相互作用や新規の結合タンパク質が同定出来る。
3) 間接免疫抗体法でRad51タンパク質の細胞内発現や染色体上のDNA損傷部位への局在(foci formation)を調べることが出来る。
4)Chromatin Immunoprecipitation

性状:0.09% アジ化ナトリウムを含む抗血清
 
背景:出芽酵母のRad51タンパク質は大腸菌のRecAタンパク質やヒトRad51タンパク質の機能的及び構造的ホモログであって、相同的組換え及び組換え修復において中心的な役割を果たし、相同なDNA鎖の交換反応を促進する機能をもっている。Rad51のパラログとして、Dmc1Rad55 Rad57などが知られておりこれらはRad51やRad52と複合体を形成して、組換え反応に関与している。
 
 
商品コード
容量
価格
 
62-101
100 ul
39,000円(税抜)

製品名
 
製品簡略説明
商品コード:62-029

Med9 is a component of the Mediator complex a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex having a compact conformation in its free form is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins
Med9 consists of 149 amino acids with molecular mass of 17376 Da
 
 
商品コード
容量
価格
 
62-029
100 ul
39,000円(税抜)

製品名
 
製品簡略説明
製品番号: 62-030

Background: Component of the Mediator complex a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. The Mediator complex is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex (PIC) with RNA polymerase II and the general transcription factors. The essential MED11/22 heterodimer specifically functions in promoting stable PIC formation
 
 
商品コード
容量
価格
 
62-030
100 ug
39,000円(税抜)

分子遺伝学 > DNA組換え
製品名
 
製品簡略説明
63-001

反応特異性検定:rhp51欠損分裂酵母株抽出液ではWBで反応しない

保存温度:4℃で輸送、小分けして-20℃で保存

反応性:Schizosaccharomyces pombe (分裂酵母)、Schizosaccharomyces japonica

免疫原:大腸菌で発現させて精製した全長のRhp51タンパク質

用途
1)ウェスタンブロッテイング (1000~5000倍希釈)
2)免疫沈降実験
3)Chromatin Immuno-Precipitation (ChIP)
4)免疫蛍光染色 (フォーカス観察)

性状:0.09%アジ化ナトリウム添加抗血清

背景:分裂酵母のRhp51タンパク室は大腸菌のRecAタンパク質やヒトRad51タンパク質の機能的及び構造的ホモログであって、相同的組換え及び組換え修復において中心的な役割を果たし、相同なDNA鎖の交換反応を促進する機能をもっている。

 
 
商品コード
容量
価格
 
63-001
100 ul
39,000円(税抜)

製品名
 
製品簡略説明
Code:63-030

Background: Psf1 is a component of GINS complex which is required for the initiation and elongation stages of DNA replication. Psf1 is required for maintenance, as well as establishment of Cdc45 binding and chromatin association of the Dpb4 subunit of Pol epsilon.
 
 
商品コード
容量
価格
 
63-030
100 ul
34,500円(税抜)

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