The Rad6 (UBE2B)-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 (509 aa 57.4 kDa) as a ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA.
Applications (see Ref 1~3)
1) Western blotting (1000 fold dilution).
2) Immuno-precipitation (200~500 dilution))
3) Indirect immuno-fluorescence staining. (assay dependent)
4) Immuno-histochemistry (100~300 fold dilution)