Backgroud: Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2.
Molecular processing: The toxin encoded 1315 amino acids with mass of 150682 Da which is proteolyticallly processed into Light Chain (2-457) and Heavy Chain (458-1315) upon secretion. These remain linked by a disulfide bridge and are non-toxic after separation.
1. Western blotting (1/1000 dilution)
2. ELISA (assay dependent)
3. Neutralising of the toxin activity (assay dependent)
Other applications have not been tested.
Immunogen: Tetanus toxoid (Clostridium tetani Harvard vaccine strain)
Reactivity: Reacts with Light Chain of tetanus toxin.