Registration
          
Cart
      
Infomation of Product
Sort
All topics:34 topics
1 topic~10 topics
 next
Product
 
Information
01-001

Storage: Shipped at 4°C or -20℃ and store at -20°C (-80°C for long-term storage).

Applications
1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization (1).
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

Form: 1.0 mg/ml in 20 mM Tris-HCl (pH 8.0) 1 mM EDTA 150 mM KCl1 mM DTT 50% glycerol

Purity: Over 90% by SDS-PAGE (CBB staining)

Background: E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity DNA annealing activity formation of D-loop and Holliday structure in homologous recombination reaction and coprotease activities that promote self-cleavages of LexA repressor lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its homologs in eukaryotes are Rad51 protein and Dmcl protein (2).
 
 
Code
Quantity
                  Price
 
01-001
100 ug
95 USD

Molecular Genetics > DNA Repair
Product
 
Information
01-007 01-008

Product: Recombinant RuvA protein with no tag attached
Function:Holliday junction-specific binding protein. Forms a complex
with RuvB motor protein and together promotes branch
migration of Holliday junction in late stage of homologous
recombination.
Purity: RuvA protein over 90% by SDS-PAGE (CBB staining)
Concentration: 2.7 mg/ml (determined by BCA method)
Form: 50% glycerol 10 mM Tris-HCl (pH7.5) 2 mM EDTA 100 mM NaCl
5 mM mercaptoethanol
Storage: -20C (long period -80C)
 
 
Code
Quantity
                  Price
 
01-007
20 ug
420 USD
01-008
100 ug
1,260 USD

Product
 
Information
Code:10-001、10-002

Human Rad51 protein is a functional and structural homolog of E. coli RecA protein which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1). Rad51 functionally and physically interacts with its paralogs Dmc1 Rad51B Rad51C Rad51D Xrcc2 and Xrcc3 and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1 BRCA2 and P53 for the maintenance of genome stability (1).
Rad51 protein was highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein (Fig. 1). Since the tag was removed from the recombinant protein (it still contains Gly-Ser-His at the N-terminal) it has been shown to retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52 (2). This product was confirmed to possess single strand DNA stimulated ATPase activity.

Applications
1) Studies on homologous recombination in mammals including human
2) Studies on the interaction of Rad51 protein with various proteins
3) To be used as a standard for Western blotting
 
 
Code
Quantity
                  Price
 
10-001
20 ug
420 USD
10-002
100 ug
1,260 USD

Product
 
Information
01-009 01-010

Product: Recombinant RuvB protein with no tag attached
Function: A motor protein for Holliday junction branch migration.
Forms a complex with RuvA Holliday-junction binding
protein and together promotes branch migration of
Holliday junction in late stage of homologous
recombination.
Purity: RuvB protein over 90% by SDS-PAGE (CBB staining)
Concentration: 1.0 mg/ml (determined by BCA method)
Form: 50% glycerol 10 mM Tris-HCl (pH7.5) 2 mM EDTA 100 mM NaCl
5 mM mercaptoethanol
Storage: -20C (long period -80C)
 
 
Code
Quantity
                  Price
 
01-009
20 ug
420 USD
01-010
100 ug
1,260 USD

Product
 
Information
01-011 01-012

Backgounds:RuvC protein of E. coli resolves the recombination intermediate Holliday junction in late stage of homologous recombination and recombination repair

Product: Recombinant RuvC protein with no tag attached

Function: Holliday junction (homologous DNA duplexes held by single strands cross-over) specific endonuclease.

Purity: RuvC protein over 90% by SDS-PAGE (CBB staining)

Concentration: 2.0 mg/ml (determined by BCA method)

Form: 50% glycerol 10 mM Tris-HCl (pH7.5) 2 mM EDTA 100 mM NaCl
5 mM mercaptoethanol
 
 
Code
Quantity
                  Price
 
01-011
20 ug
420 USD
01-012
100 ug
1,260 USD

Product
 
Information
Code:10-003、10-004

Backgrounds:Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands (1). Rad52 functionally and physically interacts with Rad51 Dmc1 and RPA in recombination processes. In radiation damaged cell Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and forms nuclear foci

Product:Expressed as a tagged protein in E. coli and highly purified

Form: 1 mg/ml in 20 mM Tris-HCl pH8.0
   100 mM KCl 2 mM 2ME 0.5 mM EDTA 20% glycerol

Reference: This product was used in reference 3
1. Friedberg EC et al. DNA Repair and Mutagenesis 2nd ed. ASM Press
2. Kitao H & Yuan Z M J. Biol. Chem. 277:48944 (2002)
3. Kurumizaka H et al. J Biol Chem 276: 35201

Related Products:
70-011 anti-Rad52 (human) rabbit affinity purified antibody
10-001 Rad51 (human) protein
70-001 Anti-Rad51 (human) rabbit antiserum
 
 
Code
Quantity
                  Price
 
10-003
20 ug
420 USD
10-004
100 ug
1,260 USD

Virology > SARS Virus
Product
 
Information
Code:65-101

Background:A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by infecting mouse with SARS virus and as the clone which produces the antibody that specifically reacts with the virus-infected cell in the laboratory of Prof. K. Ikuta of Osaka University. Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains.
Applications
1) Western blotting (0.1~0.3 μg/ml) 2) Immunofluoresece
staining (IHC) 3) ELISA
Isotype: IgG2b (kappa)
Form: Purified IgG 1 mg/ml in PBS (-) 50% glycerol filter-
sterilized azide free

References: This product has been used in references 2.
1. Satija N Lal SK The molecular Biology of SARS coronavirus. Ann. N.
Y. Acad. Sci. 1102 26-38 (2007)
2. Yamate M. et al. Establishing of Vero E6 cell clones persistently
infected with severe acute respiratory syndrome coronavirus.
Microbes and Infection 7:1530-1540 (2005)
 
 
Code
Quantity
                  Price
 
65-101
50 μg
210 USD

Product
 
Information
Code:65-901

Prion protein PrP is a membrane glycosylphosphatidylinositol(GPI) anchored glycoprotein highly expressed in neuron and glia cells as well as immune and reproductive cells. Mutations in the octapeptide repeat regions as well as elsewhere in this gene have been associated with neurodegenerative diseases such as Creutzfeldt Jakob disease fatal familial insomnia Gerstmann Straussler disease Huntington disease like 1 and kuru. The infectious isoform of PrPC known as PrPSc is able to convert normal PrPC proteins into the infectious isoform which is insoluble amyloid aggregate by changing their conformation (1).
Mature PrP protein in human consists of 209 amino acids. Several topological forms exist; one cell surface form anchored via glycolipid and two transmembrane forms therefore multiple bands are observed in SDS-PAGE (Figure).
Applications
1) Western blotting (~0.5 μg/ml) 2) ELISA (most suitable)
Other applications have not been tested.  
 
 
Code
Quantity
                  Price
 
65-901
50 μg
210 USD

Product
 
Information
Code:65-903

Prion protein PrP is a membrane glycosylphosphatidylinositol(GPI) anchored glycoprotein highly expressed in neuron and glia cells as well as immune and reproductive cells. Mutations in the octapeptide repeat regions as well as elsewhere in this gene have been associated with neurodegenerative diseases such as Creutzfeldt Jakob disease fatal familial insomnia Gerstmann Straussler disease Huntington disease like 1 and kuru. The infectious isoform of PrPC known as PrPSc is able to convert normal PrPC proteins into the infectious isoform which is insoluble amyloid aggregate by changing their conformation (1).
Mature PrP protein in human consists of 209 amino acids. Several topological forms exist; one cell surface form anchored via glycolipid and two transmembrane forms which are responsible for appearance of multiple bands in SDS-PAGE (Figure). 
Applications
1) Western blotting (~0.5 μg/ml) 2) ELISA
Other applications have not been tested.  
 
 
Code
Quantity
                  Price
 
65-903
50 μg
210 USD

Product
 
Information
Code:02-040、02-040-5

T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity (12). It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
Applications: 1. Promoting DNA replication and recombination by stabilizing single-stranded DNA (1)
2. Increase specificity and yields of long PCR products (3)
Quality Assurance: Greater than 95% of protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
Form: 10 mg/ml in 20mM Tris-HCl (pH 8.0) 100mM NaCl 0.5mM dithiothreitol 1mM EDTA 50% glycerol
Storage: -20℃
 
 
Code
Quantity
                  Price
 
02-040
200 ug
100 USD
02-040-5
5 x 200 ug
345 USD

 next
About Security  |   Policy  |   Escape  |   Site map  |   Address
Copyright(C) BioAcademia, Inc. 2006. All Rights Reserved.