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Code:70-310


Applications:
1) Western blotting (Fig. 1) 2) Immunocytochemistry (Fig. 2) 3)ELISA 4) Dot blotting

Properties: When injected into the cytoplasm, this antibody accumulates
into the nuclear pores of HeLa cells and inhibits nuclear localization
of endogenous Ran.

Immunogen: Recombinant GST-fused human Nup98 (amino acids 1-466)

Isotype: Rat IgG2c (κ)

Reactivity: Human, mouse, rat and S. pombe Nup98 proteins. S. cerevisia
nucleoporins, Nup116, Nup100, Nup145N, Nup57, Nup49. This antibody does
not react with Tetrahymena. Other species have not been tested.

Form: Purified IgG (1 mg/ml) in PBS(-), 50% glycerol, filter-sterilized.
Azide and carrier free

Storage: Ship at 4℃ or -20℃, and upon arrival, spin-down and store at
-20℃.

 
 
Code
Quantity
                  Price
 
70-310
100 ug
345 USD

Product
 
Information
Code:70-345

Storage: Shipped at 4℃ or -20℃, and upon arrival, spin-down and store at -20℃.

Reactivity: Human, Tetrahymena, S. cerevisiae, and S. pombe.. Not tested in other species

Immunogen: Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664

Applications: See Table 1 for summary. For details read Ref.1
1) Clone 13C2 antibody is most suitable for Western blotting, in human, Tetrahymena ,S. cerevisiae, and S. pombe. Not tested in other species.
2) Clone 21A10 antibody is most suitable for Immunofluorescence staining / Immunocytochemistry in human, T.etrahymena, S. cerevisiae, and S. pombe. Not tested in other species.

Epitope determined: FGxxN for clone 13C2 and GLF for clone 21A10

Isotype: Mouse IgG1 (κ) for both clones.

Form: Protein A affinity-purified IgG, 1 mg/ml in PBS(-), 50% glycerol, filter-sterilized. Azide- and carrier- free
 
 
Code
Quantity
                  Price
 
70-345
100 ug
345 USD

Product
 
Information
Code:70-346

Storage: Shipped at 4℃ or -20℃, and upon arrival, spin-down and store at -20℃.

Reactivity: Human, Mouse, S. cerevisiae, S. pombe and Tetrahymena. Not tested in other species

Immunogen: Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664

Applications: See Table 1 for summary. For details read Ref.1
1) Western blotting, (S. cerevisiae, S. pombe,) Not suitable for human. Clone 13C2 is recommended for WB in human Tetrahymena and yeasts.
2) Most suitable for Immunofluorescence staining / Immunocytochemistry for human, mouse, Tetrahymena, S. cerevisiae, S. pombe.

Epitope determined: GLF (Ref.1)

Isotype: Mouse IgG1 (κ)

Form: Purified IgG, 1 mg/ml in PBS(-), 50% glycerol, filter-sterilized. Azide- and carrier- free
 
 
Code
Quantity
                  Price
 
70-346
100 ug
345 USD

Product
 
Information
Code:70-347

Storage: Shipped at 4℃ or -20℃, and upon arrival, spin-down and store at -20℃.

Reactivity: Human, Tetrahymena, S. cerevisiae, and S. pombe.. Not tested in other species

Immunogen: Synthetic peptides containing conserved N-terminal
sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664

Applications: See Table 1 for summary. For details read Ref.1
1) Clone 13C2 antibody is most suitable for Western blotting, in human, Tetrahymena ,S. cerevisiae, and S. pombe. Not tested in other species.
2) Clone 21A10 antibody is most suitable for Immunofluorescence staining / Immunocytochemistry in human, T.etrahymena, S. cerevisiae, and S. pombe. Not tested in other species.

Epitope determined: FGxxN for clone 13C2 and GLF for clone 21A10

Isotype: Mouse IgG1 (κ) for both clones.

Form: Protein A affinity-purified IgG, 1 mg/ml in PBS(-), 50% glycerol, filter-sterilized. Azide- and carrier- free
 
 
Code
Quantity
                  Price
 
70-347
50 + 50 ug
345 USD

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