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Immunology > Immunoregulation
Product
 
Information
72-007

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human

Immunogen: Recombinant extracellular portion of human FcεR1α (corresponding to amino acids Met-26-197 where signal peptide is 1-25)

Epitope: Amino acids 110-197 of FcεR1α

Applications:
1) Western blotting (~1ug/ml)
2) Flow-Cytometry
3) Immunohistochemistry (Paraffin and Frozen) and immunocytochemistry
4) Titration of IgE-bound fraction of the FcεR1α using CRA1 and CRA2 antibodies

Isotype: IgG1 (κ)

Purity: This product is the IgG fraction purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions.

Form: 1mg/ml in PBS (pH 7.4) 50% glycerol filter-sterilized azide and carrier free. Biotinylated


Background:FcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one α one β and two γ subunits. The latter two subunits are required for signal transduction activity. The FcεR1αcomplex plays an important role in triggering allergic responses.
The CRA2 (AER24) monoclonal antibody reacts with the FcεR1αsubunit on a region that overlaps the region of the IgE binding site thus it competes with IgE for the receptor binding. Since the CRA1 (AER37) monoclonal antibody reacts with the site different from the IgE binding site on FcεR1α it does not compete with IgE for the receptor binding. Combining the two antibodies one can quantitatively measure the amounts of the IgE-bound FcεR1α.

 
 
Code
Quantity
                  Price
 
72-007
50 ug
330 USD

Immunology > Immunoregulation
Product
 
Information
72-003

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human house musk shrew

Immunogen: Recombinant extracellular portion of human FcεR1α (corresponding to amino acids Met-26-197 where signal peptide is 1-25)
Epitope: 26-110 amino acids

Applications:
1) Western blotting (~1ug/ml)
2) Flow Cytometry (FC) (1~5 ug/ml)
3) IHC-P IHC-F (~1 ug/ml)
4) Titration of IgE-bound receptor in combination with CRA2 antibody (Ref.3)
5) ELISA

Isotype: IgG2b

Form: Biotynylated monoclonal antibody (IgG) 1.6 mg/ml (depends on Lot) in PBS (pH 7.4) 50% glycerol filter-sterilized azide free
 
 
Code
Quantity
                  Price
 
72-003
50 ug
345 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-503

Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor HB-EGF (1 2). The former functions as juxtacrine and the latter as paracrine growth factor. HB-EGF activates EGFR and ErbB4 and promotes the development of many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin (3). Non-toxic mutant of diphtheria toxin CRM197 inhibit HB-EGF function which is elevated in most ovarian cancer is being tested as an anticancer drug (4).

Applications
1)Western blotting (0.2~1 ug /ml) 2) Immunoprecipitation.
3)Indirect immuno-fluorescence staining
Properties of the product
Antigen: Recombinant human HB-EGF ectodomain expressed in SF21 cell.
Antibody: Produced in serum-free medium and purified by combination of chromatography
Isotype: IgG1(mouse) Epitope: EGF domain Reactivity: React with human but not with mouse
Form: Biotinylated IgG (biotin/IgG = 7.5) 0.6 mg/ml in PBS 50% glycerol filter-sterilized azide-free

Reference:
1. Higashiyama S. et al. Science 251: 936 (1991)
2. Prenzel N. et al. Nature 402: 884 (1999)
3. Iwamoto R. et al. EMBO J 13: 2322 (1994)
4. Miyamoto S. et al. Cancer Res. 64:5720 (2004)

Related product: # 71-501 Anti-HB-EGF (human) antibody monoclonal (4G10). # 01-515 Diphtheria toxin mutant CRM197. # 01-517 Diphtheria toxin..
 
 
Code
Quantity
                  Price
 
71-503
50 ug
330 USD

Product
 
Information
Product code 80-001 No Stock

This kit can measure the amount of HIV-1 Gag p24 antigen in cell culture medium handily by a sandwich ELISA (Enzyme Linked Immunosorbent Assay) method. p24 antigen is a structural protein of HIV-1 so the virus titer in the sample could be implicated from the measurement.

[Principle of the test]
This kit is based on the principle of a sandwich ELISA method that uses an antibody coated plate as a solid phase and a biotinyl antibody and a peroxidase-labeled streptavidin.

[Advantage of this kit]
○ p24 of subtype AE can be measured at the same sensitivity as subtype B because affinity purified polyclonal antibody raised against the full length recombinant p24 is used.
○ Risk-free kit because neither patient sera nor active virus products are used.
○ Assay can be performed at room temperature.

[Preservation and Expiration Date]
Storage : 2 ~ 8℃ (Please do not freeze.)
Expiration date for use : 1 year

[Required Reagent and Apparatus and Equipment]
1. Deionized water
2. Test tubes or microtubes (for sample
preparation)
3. Micropipettes and tips
4. Microplate reader
 
 
Code
Quantity
                  Price
 
80-001
96 assay
Price inquiry

Product
 
Information
Code:65-053

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 94 kb which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. HCV core protein is not only a component of nucleocapsid but also has multiple functions and is a pathogenic factor for hepatitis. It also participates in some cellular processes including transcriptional regulation and cellular transduction. HCV core antigen is used as diagnostic marker for HCV infection.

Applications
1. Western blotting 2. Immunohistochemistry 3. Immuno-fluorescence staining
4. ELISA 5. FACS

Immunogen: A part of the core region (nucleotides 369-704 amino acids 13-124) of HCV genotype 1b expressed in E. coli

Conjugate: Bitin conjugated [biotin] / [IgG] = 6.6

Isotype: Mouse IgG2a kappa

Form: 0.7 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV core antigen of genotype 1b. Not tested in other genotype
 
 
Code
Quantity
                  Price
 
65-053
50 ug
315 USD

Product
 
Information
Code:65-058

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of hepatitis. The polyprotein encoded is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. NS4a protein (54 amino acids) forms a comlex with NS3 and functions as a cofactor for NS3 protease activity.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS4 protein (the nucleotide sequence is shown in ref.4) of HCV genotype 1b expressed in E. coli. The epitope of this antibody was mapped to the N-terminal region of the NS4 protein (NS4a).

Conjugate: Biotin conjugated [biotin] / [IgG] = 11.3

Isotype: Mouse IgG2b kappa

Form: 0.6 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS4a protein of genotype 1b. Not tested in other genotypes.

 
 
Code
Quantity
                  Price
 
65-058
50 ug
310 USD

Product
 
Information
Code:65-063

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. The primary function of NS5a is not known but from the comparative studies with other viruses it is predicted to play a role in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. FACS

Immunogen: A region of NS5a protein of HCV genotype 1b (ref.4) expressed in E. coli

Conjugate: Biotin conjugated [biotin] / [IgG] = 9.6

Isotype: Mouse IgG 2a kappa

Form: 0.8 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5a protein of genotype 1b. Not tested in other genotypes.
 
 
Code
Quantity
                  Price
 
65-063
50 ug
315 USD

Product
 
Information
Code:65-068

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of hepatitis. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS5b protein of HCV genotype 1b expressed in E. coli

Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

Isotype: Mouse IgG2b kappa

Form: 0.7 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5b protein of genotype 1b. Not tested in other genotypes.
 
 
Code
Quantity
                  Price
 
65-068
50 ug
315 USD

Product
 
Information
Code:72-031

Storage and Shipment: Shipped at 4℃ and stored at -20℃. Do not freeze below -20℃

Reactivity: Human

Immunogen: Recombinant extracellular domain of CD40

Applications:
1. Flowcytometry
2. Immunohistochemistry (acetone-fixed frozen section; indirect immuno-staining)

Isotype: Mouse IgG1κ

Form: 0.75~1.0 mg/ml in PBS, 50% glycerol, filter-sterilized
 
 
Code
Quantity
                  Price
 
72-031
50 ug
210 USD

Product
 
Information
Code:65-021

HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). p24 is used as a marker antigen for observing the patient’s condition after treatment as it indicates the amount of virus in the blood.
The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus subtype B (2) and was highly purified by several steps of chromatography (3 4).
Using this antiserum in Western blotting the bands of 24 kD 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells (Fig. 1).
This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

Applications
1. Western blotting 2. Immunoprecipitaiton 3. Immunofluorescence  staining 4. ELISA          Not tested in other application

Form: Biotin conjugated IgG (0.9 mg/ml) in PBS 50% glycerol filter-sterilized
Storage: Sent at 4℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
65-021
100 ug
420 USD

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