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Code:60-051

His6-epitope tag is especially useful because the tagged recombinant proteins can be easily purified by
affinity chromatography with metal ion (e.g.Ni2+ Co2+ etc.)) conjugated resins. Due to their small size
His epitope tag does not affect the tagged protein’s biochemical properties in most cases.
Anti-His epitope tag antibody is useful for identification of a recombinant protein tagged with 6X
His at N-terminal C-terminal and internal of the proteins.

Applications::::
1. Western blotting (dilution: 1/1000)
2. ELISA (assay dependent)
Not good for immunoprecipitation. Other applications have not
been tested.

Immunogen: : : : GSHHHHHHGGC synthetic peptide cross-linked to KLH with C-terminal Cystein.

Specificity: This antibody recognizes His6-tagged fusion proteins.

Form:::: Antiserum added with 0.05% sodium azide

Storage: Shipped at 4℃ and stored at -20℃ or below

Reference:
Simpson RJ Adams PD and Golemis EA “ Basic Methods in Protein Purification and
Analysis.A laboratory Manual” ColdSpring Harbor Laboratory Press (2009) New York
 
 
Code
Quantity
                  Price
 
60-051
100ul
220 USD

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