Registration
          
Cart
      
Infomation of Product
Sort
All topics:2 topics
1 topic~2 topics
Product
 
Information
Code:71-131

Storage temperature: Ship at 4℃ and store at -20℃.

Immunogen: synthetic peptide containing acetyl-Lys315 of human p53

Isotype: mouse IgG1

Applications
1. Western blotting (~1 ug/ml) (Fig.1)
2. Immuno-precipitation (Fig.2)
3. Immunofluorescence staining (Fig.3)
4. Flow-Cytmetry (1/100)
5. ELISA

Form: purified monoclonal antibody (IgG) 1mg/ml in PBS (pH 7.4) 50% glycerol

Isotype: mouse IgG1 (κ)

Background: p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule.
Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60 and activates transcription of proapoptotic genes BAX and PUMA .
 
 
Code
Quantity
                  Price
 
71-131
100 ug
345 USD

Molecular Biology / Cell Biology > Apoptosis
Product
 
Information
Code:71-133

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule.
Acetylation of lysine 382 (acetyl-K382) of p53 occurs after DNA damage and is catalyzed by the p300/CBP acetyltransferase which stabilizes p53 proten (ref 1).
 
 
Code
Quantity
                  Price
 
71-133
100 ug
345 USD

About Security  |   Policy  |   Escape  |   Site map  |   Address
Copyright(C) BioAcademia, Inc. 2006. All Rights Reserved.