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Product
 
Information
01-003 01-004

Storage: Ship with ice-pack or at -20℃ and store at -20℃ or -80℃ (long period storage)

Product:Highly purified recombinant protein (>95%)

Form: 0.2~0.5 mg/ml in 20 mM Tris-HCl (pH 7.5) 1 mM EDTA 50 mM KCl 1 mM DTT 50% glycerol


Backgroud: RecQ DNA helicase of E. coli is the prototype of human
RecQ-like helicases such as Bloom and Werner hericases whose
defect causes predisposition to cancer and premature senescence.


 
 
Code
Quantity
                  Price
 
01-003
20 ug
420 USD
01-004
100 ug
1,680 USD

Molecular Genetics > DNA Replication
Product
 
Information
Code:10-101

Backgrounds:DNA polymeraseβ is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized methylated deaminated etc.) (ref. 1).This product is highly purified full-length rat DNA polymeraseβ overproduced in E. coli with high enzymatic activity without any tag attached (ref.2).

Applications
1) For the studies on the mechanisms of base-excision repair of DNA damage
2) As a positive control of western blotting with anti-DNA polymeraseβ antibody

Properties of the product
Enzyme activity: 90 unit/ul (1unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37℃ in 60 min.)
Purity: Over 95% pure by SDS-PAGE analysis
Form: 1.3 mg/ml in 50mM Tris-HCl pH7.6 0.3M KCl 0.1mM EDTA 1mM DTT 20% glycerol

References: This product is described in ref. 2
1. Friedberg EC et al. DNA Repair and Mutagenesis 2nd ed. ASM Press (2006)
2. Date T. et al. Biochemistry 27: 2983 (1988)

Related product
#70-041 anti-DNA polymeraseβ (rat) antibody affinity purified cross-reacts with human and mouse homologs.
 
 
Code
Quantity
                  Price
 
10-101
20 ug
315 USD
10-102
100 ug
945 USD

Product
 
Information
02-001 02-001-5

Thermus aquaticus DNA polymerase (Taq DNA polymerase) has
thermostable DNA polymerase activity is suitable for PCR reactions.

Applications:
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP dITP and
fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine)
at the 3’-blunt ends

Form;20mM Tris-HCl (pH 8.0) 100mM KCl 0.1mM EDTA 1mM DTT 50% glycerol
0.5% Tween20 0.5% Igepal CA-630.

Store at -20℃
Concentration:5 units/ul
 
 
Code
Quantity
                  Price
 
02-001
200 U
40 USD
02-001-5
5 x 200 U
160 USD

Product
 
Information
02-011 02-011-5

Thermus aquaticus DNA polymerase (Taq DNA polymerase) has thermostable DNA polymerase activity and suitable for PCR.

Applications:
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP dITP and
fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine)
at the 3’-blunt ends

Storage Conditions:Ship at 4℃ or -20℃ and store at -20℃

Form: 20mM Tris-HCl (pH 8.0) 100mM KCl 0.1mM EDTA 1mM DTT 50% glycerol
0.5% Tween20 0.5% Igepal CA-630 Store at -20℃

Concentration: 5 units/ul
 
 
Code
Quantity
                  Price
 
02-011
200 U
30 USD
02-011-5
5 x 200 U
120 USD

Product
 
Information
Code:02-021、02-021-5

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa same as that of the natural Pfu DNA polymerase.
■ Pfu DNA polymerase is thermostabe and has low error rates.
■ It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
■ Pfu DNA polymerase-generated PCR
fragments are blunt-ended.
Applications:
1) cloning
2) DNA expression
3) site-directed mutagenesis
Storage Conditions:
50mM Tris-HCl (pH 8.2) 0.1mM EDTA 1mM DTT 50% glycerol 0.1% Tween20 0.1% Igepal CA-630 Store at -20℃
Concentration:2.5 units/l where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72℃ when activated salmon sperm DNA was used as template/primer.
 
 
Code
Quantity
                  Price
 
02-021
200 U
62 USD
02-021-5
5 x 200 U
248 USD

Product
 
Information
Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa same as that of the natural Pfu DNA polymerase.
■ Pfu DNA polymerase is thermostabe and has low error rates.
■ It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
■ Pfu DNA polymerase-generated PCR
fragments are blunt-ended.
Applications:
1) cloning
2) DNA expression
3) site-directed mutagenesis
Storage Conditions:
50mM Tris-HCl (pH 8.2) 0.1mM EDTA 1mM DTT 50% glycerol 0.1% Tween20 0.1% Igepal CA-630
Store at -20℃
Concentration:2.5 units/l where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72℃ when activated salmon sperm DNA was used as template/primer.
 
 
Code
Quantity
                  Price
 
02-031
200 U
50 USD
02-031-5
5 x 200U
200 USD

Molecular Genetics > DNA Repair
Product
 
Information
70-041

Reactivity: human rat mouse hamster

Immunogen: Recombinant rat DNA polymerase beta functional

Applications
1) Western blotting (1/ 1000~ 1/3000)
2) Immunoprecipitation (1/100)
3) Immunofluorescent staining (1/1000)
4) ELISA (assay dependent)

Form: 1 mg/ml in PBS 50% glycerol filter-sterilized. Azide and carrier free



 
 
Code
Quantity
                  Price
 
70-041
50 ug
345 USD

Molecular Genetics > DNA Replication
Product
 
Information
Code:70-056

Applications:
1) Western blotting (1/2,000 dilution
2) Immunoprecipitation (Assay dependent)
Other applications have not been tested.

Antigen: Recombinant human p66 subunit of DNA polymerase delta

Reactivity: Human p66 protein. Not tested with other species

Isotype: IgG2B (kappa)

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS (pH 7.4), 50%
glycerol, sterile-filtered, azide free

Storage: Shipped at 4℃ or -20℃ and stored at -20℃.
 
 
Code
Quantity
                  Price
 
70-056
100 ug
Price inquiry

Product
 
Information
code:02-721

This cDNA library (plasmid DNA) is constructed from Human Umbilical Vein Endothelial Cell (HUVEC)-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express human genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
 
 
Code
Quantity
                  Price
 
02-721
500 ng
330 USD

Product
 
Information
Code:02-713

This cDNA library (plasmid DNA) is constructed from mouse thymocyte-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express genes in mammalian cells as it contains SV40 promoter. It also contains f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoter for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
 
 
Code
Quantity
                  Price
 
02-713
500 ng
330 USD

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