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62-003

Storage: Sent at 4 ℃ and store at -20℃
Reactivity: S. pombe Gcn5 protein
Immunogen: Recombinant Gcn5 protein (His-tagged 1-300 amino acids)

Applications: Western blotting.  (Not tested for other applications)
Form: Whole rabbit antiserum added with 0.1% sodium azide

Function: AGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histone acetylase complex which has Gcn5p as a catalyst subunit and has functions overlapping with the fundamental transcription factor TFIID which has Taf1p as a catalyst subunit. However SAGA and TFIID have different allotments and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds) TBP related protein group (4 kinds) TAF protein group (5 kinds that also pertain to TFIID) and other protein groups (>6 kinds). It has the molecular functions such as chemical modification of histone recruitment by direct interaction of transcription regulating factor on DNA and control of transcription starting reaction by TBP. Gcn5p that shows histoneacetylase activity is one of the proteins belonging to the above-mentioned Ada protein group and it is composed of 439 amino acid recidues with molecular mass of 51 kDa in the case of budding yeast.
 
 
Code
Quantity
                  Price
 
62-003
100 ul
345 USD

Molecular Genetics > Transcription・Translation
Product
 
Information
62-009

Storage temperature: Ship at 4℃and store at -20℃

Reactivity: S. cerevisiae Sua7 / TFIIB protein

Immunogen: Rcombinant His-tagged full-size Sua7 protein

Applications
1.Western blotting. (1/1000~1/5000)
2.Immunoprecipitation
3.Chromatin Immuno-Precipitation
4.ELISA

Form: 0.1% sodium azide added to the antiserum

Background: The fundamental transcription factor TFIIB has the characteristics of stabilizing the DNA binding of TATA box-binding protein (TBP) and binding directly to DNA by its conformational change. Also its N terminal region binds to the RNA channel of RNA polymerase undertaking a very important role in the determination of transcription initiation point and promoter clearance. Sua7p is the TFIIB of budding yeast and is composed of 346 amino acid residues (aa)
 
 
Code
Quantity
                  Price
 
62-009
100 ul
345 USD

Product
 
Information
62-011

Storage temperature: Ship at 4℃ and store at -20℃

Reactivity: S. cerevisiae Taf4 protein.

Immunogen: Recombinant His-tagged N-terminal domain of Taf4p protein (1-200aa)

Applications
1. Western blotting. (1/1000~1/5000 dilution)

Form: Whole antiserum added with 0.1% sodium azide added to the antiserum

Background: The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf4p is one of the subunits of TFIID and in the case of budding yeast it is composed of 388 amino acid residues (aa). This protein contains histone folds in its interior and forms TAF octamer with Taf6p Taf9p and Taf12p.

 
 
Code
Quantity
                  Price
 
62-011
100 ul
345 USD

Product
 
Information
62-013

Storage: Ship at 4℃ and store at -20℃

Reactivity: S. cerevisiae Taf6 protein.

Immunogen: Recombinant His tagged Taf6 protein (1-200 aa)

Applications: Western blotting (1/1000)

Form: Whole antiserum added with 0.1% sodium azide

Background: The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf6p is one of the subunits of TFIID and in the case of budding yeast it is composed of 516 amino acid residues (aa). Taf6p is also a subunit of histoneacetylase complex SAGA which is said to have an overlapping function with TFIID. The protein contains histone folds in its interior and forms TAF octamer together with Taf4p Taf9p and Taf12p.
 
 
Code
Quantity
                  Price
 
62-013
100 ul
345 USD

Product
 
Information
Code:62-015

The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf10p is one of the subunits of TFIID and in the case of budding yeast it is composed of 206 amino acid residues (23 kDa) . Taf10p is also a subunit of histone acetylase complex SAGA which is said to have an overlapping function with TFIID. This protein contains histone folds in its interior and forms dimers with Taf3p and Taf8p each.
 
 
Code
Quantity
                  Price
 
62-015
100 ul
345 USD

Product
 
Information
Code:62-017

The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf11 is one of the subunits of TFIID and in the case of budding yeast it is composed of 346 amino acid residues with molecular mass of 40624.
 
 
Code
Quantity
                  Price
 
62-017
100 ul
345 USD

Product
 
Information
Code:62-021

Storage: Ship at 4℃ and store at -20℃

Reactivity: S. cerevisiae Toa1 protein.

Immunogen: Recombinant His-tagged Toa1 protein (1-286aa) produced in E. coli

Applications: Western blotting (1/1000~1/5000)

Form: Whole antiserum added with 0.1% sodium azide

Background:The fundamental transcription factor TFIIA stabilizes the DNA binding of TATA box-binding protein (TBP). It also plays a very important role in the activation of fundamental transcription factor TFIID by the function of the transcription regulator factor. The budding yeast TFIIA is composed of two subunits Toa1p (286aa) and Toa2p (122aa).

 
 
Code
Quantity
                  Price
 
62-021
100 ul
345 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
71-001

Storage : Shipped at 4℃ and store at -20℃

Validation: Indpendently validated in Ref.3 for IF.

Reactivity: human monkey rodents dog

Immunogen: Recombinant human RanBP9 from Phe133 to Tyr 229.

Applications
1. Western blotting (1/1000- 1/ 2000 dilution)
2. Immunoprecipitation (assay dependent concentration)
3. Immunofluorescence stainig (1/200~1/1000 dilution)
4. Immunohistochemistry (1/200 dilution. Perform heat mediated
antigen retrieval with citrate buffer (pH 6) before formalin
treated paraffin embedded sectioning)

Form: Undiluted antiserum added with 0.09% sodium azide

Background: RanBPM (RanBP9) was identified as a protein that interacts with small GTP-binding protein Ran and forms a 670 kD multiprotein complex. The protein consists of 729 amino acids and is identified by Western blotting as an apparent molecular mass of 95 kD (see below). It is involved in nucleation of microtubules and controls cell growth by interacting with many protein factors.
 
 
Code
Quantity
                  Price
 
71-001
100 ul
330 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-003
Storage : Shipped at 4℃ and store at -20℃
Reactivity: human monkey rodents dog
Immunogen: Recombinant human RanBP9 from Phe133 to Tyr 229.

Applications
1. Western blotting (1/1000- 1/ 2000 dilution)
2. Immunoprecipitation (assay dependent concentration)
3. Immunofluorescence stainig (1/200~1/1000 dilution)

Form: Undiluted antiserum added with 0.09% sodium azide

Background: RanBPM (RanBP9) was identified as a protein that interacts with small GTP-binding protein Ran and forms a 670 kD multiprotein complex.
 
 
Code
Quantity
                  Price
 
71-003
100 ul
330 USD

Molecular Biology / Cell Biology > Apoptosis
Product
 
Information
Code:71-133

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule.
Acetylation of lysine 382 (acetyl-K382) of p53 occurs after DNA damage and is catalyzed by the p300/CBP acetyltransferase which stabilizes p53 proten (ref 1).
 
 
Code
Quantity
                  Price
 
71-133
100 ug
345 USD

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