Registration
          
Cart
      
Infomation of Product
Sort
All topics:2 topics
1 topic~2 topics
Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
01-531

Storage: Ship at 4℃ or -20℃, and store at -20℃ (For longer period,  years, store at -80℃)

Product: Recombinant streptolysin O. Functional in membrane pore   formation to introduce molecules into living animal cells. The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci (His6-tagged to the signal peptide removed N-terminal of SLO). The specific activity is as high as 1,200,000- 2,000,000 hemolytic units (HU) /mg (depending on Lot). 

Applications
1) Functional studies
2).Reagent for membrane pore formation to introduce small-to-   macromolecules into living cells (For protocol refer to or other references; Walev I et al “Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.” PNAS 98: 3185-3190 (2001) PMID: 11248053)

3).Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent), ELISA
4) Western blotting, Dot blotting,
5) Immuno-chromatography
6) SDS-PAGE

Measurement of the activity: Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37℃ for 30 min.

Purity: Over 98% by SDS-PAGE (see Fig.1)

Form: 1 mg/ml in PBS (-), 1 mM DTT, 50% glycerol, sterilized by filtration.
 
 
Code
Quantity
                  Price
 
01-531
20 ug
290 USD
01-531-5
5 x 20 ug
870 USD

Bacteriology / Bacterial Toxin Proteins > Bacterial Toxins
Product
 
Information
Code:64-001

Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. In the hemolytic streptococci infection the antibodies to various proteins secreted from the bacteria such as toxins increase. For the diagnostic confirmation of SLO production by the infection the measurement of the antibody ASLO (Anti-Streptolysin O) is most widely used.

Applications
1) Western blotting (x2000~10000 dilution)
2) Neutralization of hemolysis by Streptolysin O (SLO)
3) Immunoprecipitaion
4) ELISA

*Most of the anti-Streptolysin O polyclonal antibodies (ASLO) sold in the current market were obtained by immunizing with SLO purified from the culture supernatant of hemolytic Streptococci. But the SLO preparations were contaminated with other secreted proteins such as NADase. This product is highly specific to SLO because of the purity of immunogen

Immunogen: His6-tagged full-size recombinant SLO of S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli

Reactivity: Streptolysin O of Group ACand G origins

Form: Undiluted serum added with 0.09 % sodium azide.

Storage: Shipped at 4℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
64-001
100 ul
345 USD

About Security  |   Policy  |   Escape  |   Site map  |   Address
Copyright(C) BioAcademia, Inc. 2006. All Rights Reserved.