Registration
          
Cart
      
Infomation of Product
Sort
All topics:2 topics
1 topic~2 topics
Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-501

Storage: Shipped at 4℃ and store at -20℃ (Do not store below -20℃)

Product: Mouse monoclonal antibody produced in serum-free medium and purified by combination of chromatography

Reactivity: React with human but not with mouse
Immunogen: Recombinant human HB-EGF ectodomain expressed in SF21 cell

Epitope: Amino acids 136-149 in the EGF domain

Applications The antibody can react with both soluble and cell-surface attached forms of HG-EGF.
1) Western blotting (0.2~1 ug /ml) non-denaturing condition.
2) Immunoprecipitation (2 ug/ml)
3) Immunofluorescence staining (5~10 ug/ml)
4) Immunohistochemistry (assay dependent)
5) Inhibition of HB-EGF ectoderm shedding (Ref 1)
6) Inhibition of Diphtheria Toxin binding to HB-EGFR (Ref 1)


Isotype: IgG1 (mouse)
Form: 1 mg/ml in PBS 50% glycerol filter-sterilized azide-free

 
 
Code
Quantity
                  Price
 
71-501
100 ug
345 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-503

Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor HB-EGF (1 2). The former functions as juxtacrine and the latter as paracrine growth factor. HB-EGF activates EGFR and ErbB4 and promotes the development of many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin (3). Non-toxic mutant of diphtheria toxin CRM197 inhibit HB-EGF function which is elevated in most ovarian cancer is being tested as an anticancer drug (4).

Applications
1)Western blotting (0.2~1 ug /ml) 2) Immunoprecipitation.
3)Indirect immuno-fluorescence staining
Properties of the product
Antigen: Recombinant human HB-EGF ectodomain expressed in SF21 cell.
Antibody: Produced in serum-free medium and purified by combination of chromatography
Isotype: IgG1(mouse) Epitope: EGF domain Reactivity: React with human but not with mouse
Form: Biotinylated IgG (biotin/IgG = 7.5) 0.6 mg/ml in PBS 50% glycerol filter-sterilized azide-free

Reference:
1. Higashiyama S. et al. Science 251: 936 (1991)
2. Prenzel N. et al. Nature 402: 884 (1999)
3. Iwamoto R. et al. EMBO J 13: 2322 (1994)
4. Miyamoto S. et al. Cancer Res. 64:5720 (2004)

Related product: # 71-501 Anti-HB-EGF (human) antibody monoclonal (4G10). # 01-515 Diphtheria toxin mutant CRM197. # 01-517 Diphtheria toxin..
 
 
Code
Quantity
                  Price
 
71-503
50 ug
330 USD

About Security  |   Policy  |   Escape  |   Site map  |   Address
Copyright(C) BioAcademia, Inc. 2006. All Rights Reserved.