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Code:70-315

The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. This hybridoma has been established by Prof. T. Tachibana’s Lab at Osaka City Univ. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.

Applications
1. Western blotting (160 kDa single band in Hela cell extract) 2. Immunocytochemistry
3. ELISA
Other applications are not tested.
Additional comments: When injected into the nucleus R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.

Specification
Immunogen: Recombinant GST-fused rat Nup153 (610-1191aa)
Epitope: 610-1191 aa (Zn finger and FG repeats domain)
Isotype: Rat IgG2aκ
Form: Purified monoclonal antibody (IgG) 1 mg/ml in PBS 50% glycerol filter-sterilized
Specificity: Specific to human mouse rat and monkey Nup153 proteins. Other species are not tested.
Storage: -20℃ (long period -70℃)

References:
1. UllmanK.S. et al. (1999) “The nucleoporin Nup153 plays a critical role in multiple types of nuclear export.” Molecular Biology of the Cell 10: 649-664.
2. LimR.Y. et al. (2007). Nanomechanical basis of selective gating by the nuclear pore complex. Science 318: 640-643.
 
 
Code
Quantity
                  Price
 
70-315
200 ug
345 USD

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