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Code:65-063

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. The primary function of NS5a is not known but from the comparative studies with other viruses it is predicted to play a role in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. FACS

Immunogen: A region of NS5a protein of HCV genotype 1b (ref.4) expressed in E. coli

Conjugate: Biotin conjugated [biotin] / [IgG] = 9.6

Isotype: Mouse IgG 2a kappa

Form: 0.8 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5a protein of genotype 1b. Not tested in other genotypes.
 
 
Code
Quantity
                  Price
 
65-063
50 ug
315 USD

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