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Code:65-021

HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). p24 is used as a marker antigen for observing the patient’s condition after treatment as it indicates the amount of virus in the blood.
The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus subtype B (2) and was highly purified by several steps of chromatography (3 4).
Using this antiserum in Western blotting the bands of 24 kD 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells (Fig. 1).
This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

Applications
1. Western blotting 2. Immunoprecipitaiton 3. Immunofluorescence  staining 4. ELISA          Not tested in other application

Form: Biotin conjugated IgG (0.9 mg/ml) in PBS 50% glycerol filter-sterilized
Storage: Sent at 4℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
65-021
100 ug
420 USD

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