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Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa and is highly conserved among eukaryotic organisms. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). Rpn7 is one of the lid subunits of the 26 S proteasome regulatory particle (1 2). The RPN7 gene is known to be essential and required for the integrity of the 26 S complex by establishing a correct lid structure (3 4).
1) Western blotting (~1000 fold dilution) 2) Immunoprecipitation 3) Not tested for other applications
Product: Rabbit polyclonal antibody affinity purified with recombinant Rpn7p
Immunogen: Recombinant yeast Rpn7p expressed in E. coli (Ref. 4)
Form: Purified IgG in 100 mM NaCl 10 mM Tris-HCl pH 7.4 0.05 % sodium azide
Reaction: S. cerevisiae Rpn7p. Not tested with other species
Storage: -20 C. To avoid repeated freezing and thawing store the antibody solution in aliquots.
Reference: This product was used in ref. 3 and 4
1. Hershko A. and Ciechanover A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67 425-479 (1998)
2. Finley D et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Isono E et al. Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae J Biol Chem. 279:27168-76 (2004)
4. Isono E et al. The assembly pathway of the 19S regulatory particle of the yeast 26S proteasomeMol. Biol. Cell 18: 569 (2007)
100 ul
400 USD

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