Infomation of Product
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1 topic~1 topics
Molecular Biology / Cell Biology > Signal Transduction
Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). Rpn5p is essential non-ATPase regulatory subunit of the 26S proteasome lid similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit Rpn7 ( 2 3). It consists of 445 amino acids with molecular weight of 51768.
1) Western blotting (~1000 fold dilution) 2) Immunoprecipitation 3) Not tested for other applications
Product: Rabbit polyclonal antibody affinity purified with recombinant Rpn5p
Immunogen: Recombinant yeast Rpn5p expressed in E. coli
Form: Purified IgG in 100 mM NaCl 10 mM Tris-HCl pH 7.4 0.05 % sodium azide
Reactivity: S. cerevisiae Rpn5p. Not tested with other species
Storage: -20℃. To avoid repeated freezing and thawing store the antibody in aliquots.
Reference: This product was used in ref. 3
1. Hershko A. and Ciechanover A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67 425-479 (1998)
2. Finley D et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Isono E et al. The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome. Mol. Biol. Cell 18: 569 (2007)
100 ul
400 USD

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