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code:10-201

Shipping and Storage: Ship with dry-ice and Store at -80℃

Product: Recombinant mouse Dnmt1 (aa 291-1620), His-Tag attached to N-
termial, expressed in baculovirus expression system.

Reagents Supplied with Enzyme 
Dnmt1 Reaction Buffer (5 x)
20mM S-adenosylmethionine (SAM) which was purified by
chromatography from the commercial reagent and dissolved in H2O
Note: SAM is very unstable. Store at -80℃

Applications
1)In vitro methylation of cytosine residues in hemimethylated
DNA at 5’….CG…3’.
2)Antigen for anti-mammalian Dnmt1 antibodies.

Form: 0.5mg protein/ml in 0.2M NaCl, 10mM HEPES (pH 7.4), 50% glycerol

Definition of specific activity: 1 unit is defined as the amount of
the enzyme that transfer 1 pmole of methyl group to poly dI-dC
substrate during 30 minutes at 37℃

Specific activity: 17 units/ul

Purity: Greater than 95% protein determined by SDS-PAGE (CBB staining)

Reaction Conditions
Incubate in 1 x Dnmt1 Reaction Buffer (20mM Tris-HCl, pH7.4, 0.5 mM
EDTA, 0.2 mM DTT, 5% glycerol) with 10μM S-adenosylmethionine (SAM)
at 37℃

 
 
Code
Quantity
                  Price
 
10-201
300 U
330 USD

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