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Information
code:03-001、03-001-5

EGF is a polypeptide growth hormone which plays an important role in the regulation of growth proliferation and differentiation of a wide range of cells by binding to its receptor EGFR (epidermal growth factor receptor). Through its binding to cell surface receptors EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT RAS/ERK and JAK/STAT pathways.
The full-length human EGF was expressed in E.coli and highly purified by the propriety chromatographic procedures. This product has the same amino acid sequence with the mature human EGF (6.35 kDa) and has no tag attached.
Applications
1. EGF is commonly used as a supplement in serum-free or reduced serum media for culture of mammalian cells.
2. Studies of the human EGF receptor transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF antibodies
4. Acceleration of the healing process of epidermis damage on skin cornea etc.
5. Widely used in cosmetics such as whitening anti-crinkle anti-aging etc.
Specification
Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96
AQueous Assay; Promega) with NHEK cells (normal human epidermal keratinocytes) was
< 0.1 ng/ml corresponding to a specific activity of < 1 x 107 units/mg.
Purity: >95% as determined by SDS-PAGE (CBB staining)
Form: 0.74 mg/ml in PBS (10mM Na-phosphate 150mM NaCl) pH7.2 50% glycerol filter-sterilyzed
Storage: -20℃ (for long-term storage -70℃)
 
 
Code
Quantity
                  Price
 
03-001
50 ug
125 USD
03-001-5
5 x 50 ug
375 USD

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