Registration
          
Cart
      
Infomation of Product
Sort
All topics:1 topics
1 topic~1 topics
Product
 
Information
code:02-701

This cDNA library (plasmid DNA) is constructed from Saccharomyces cerevisiae strain S288C- derived poly(A)+ RNA at the log phase by the Linker-Primer method (Ref.1) by Prof. H. Nojima of Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pLZ3 vector (shown below) used in this library can not replicate in S. cereviseaas but contains pUCori for replication in E. coli

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)

Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones: 3.6 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃

References
1. KoboriM. IkedaY. NaraH. KatoM. KumegawaM. NojimaH. and KawashimaH. ” Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library.” Genes Cells 3: 459-475 (1998) PMID: 9753427
2. TanakaS. and NojimaH. ”Nik1: a Nim1-like protein kinase of S. cerevisiae interacts with the Cdc28 complex and regulates cell cycle progression.” Genes Cells 1 905-921 (1996) PMID: 9077450
3. SambrookJ. and RussellDW. Molecular Cloning Chapter 11 ”Preparation of cDNA libraries and gene identification.” CSHL Press (2001)
 
 
Code
Quantity
                  Price
 
02-701
500 ng
330 USD

About Security  |   Policy  |   Escape  |   Site map  |   Address
Copyright(C) BioAcademia, Inc. 2006. All Rights Reserved.