Registration
          
Cart
      
Infomation of Product
Sort
All topics:1 topics
1 topic~1 topics
Product
 
Information
code:02-060、02-060-5

Shipping and Storage: Ship at 4℃ or -20℃ and store at -20℃

Product: Recombinant full-size functional E.coli RNase H (=RNaseHI)
over-expressed in E. coli and highly purified. MW is 17.6 kDa.

Applications
1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the
second strand of cDNA
2) Removal of poly (A) tails from mRNA after hybridization with oligo
(dT)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA

Form: 50 units/ul in 20mM Tris-HCl (pH 7.5), 100mM KCl, 1mM DTT, 50%
Glycerol
Specific Activity: ~100,000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme
that hydrolyzes 1 nmol of the
RNA in 3H-labeled M13 DNA/RNA hybrid to acid-soluble
ribonucleotides in 20 min at 37℃.

Quality Assurance: Greater than 95% protein determined by SDS-PAGE
(CBB staining)Endo- and exo-DNase activities and RNase activity were
not detected with 100 U/ml RNaseH in 50 ul reaction at 37℃.

Reagents Supplied with Enzyme: RNaseH Reaction Buffer (10 X): 100 mM
Tris-HCl (pH 8.0), 100 mM MgCl2, 500 mM NaCl,10 mM DTT, 500 ug/ml
BSA (Bovine Serum Albumin)

 
 
Code
Quantity
                  Price
 
02-060
1000 U
110 USD
02-060-5
5 x 1000 U
330 USD

About Security  |   Policy  |   Escape  |   Site map  |   Address
Copyright(C) BioAcademia, Inc. 2006. All Rights Reserved.