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Code:02-050、02-050-5

Bacteriophage T4 derived DNA ligasecatalyzes the formation of phosphodiester bonds between 3’-OH termini and 5’-P termini in duplex DNA or RNA (1). This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA RNA or DNA/RNA hybrids。
  T4 DNA ligase was expressed in E.coli in large quantities and highly purified. MW is 55.3 kDa. 
Applications:
1) Insertion of DNA fragment into a vector
2) Linker (or Adaptor) ligation with DNA fragment
Storage conditions:
10mM Tris-HCl (pH 7.6) 50mM KCl 0.1mM EDTA 1mM dithiothreitol 50% glycerol
Store at -20℃
Concentration:
400 U/l where one unit is the amount of enzyme that ligates more than 90% of 6 g of DNA-HindIII fragments in a 20l mixture in 30 minutes at 16℃.
Quality Assurance:
Greater than 95% protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
 
 
Code
Quantity
                  Price
 
02-050
20000 U
35 USD
02-050-5
5x 20000 U
130 USD

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