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Information
E05-001

Shipping and Storage: Ship at 4℃ or-20℃ and store at -20℃. Do not
freezebelow -20℃

Product: Recombinant full-size HIV-1 Reverse Transcriptase without
Tag-peptide expressed in E. coli. It is composed of heterodeimer, p66
and p51 as the one produced in HIV-1 infected cell. It is an RNA-
dependent DNA polymerase derived from HIV-1 (AIDS virus), subtype B
origin It also has RNaseH activity and is an enzyme indispensable
for reproduction of AIDS virus.

Applications
1)It is extremely effective for screening new specific inhibitors for
HIV virus as a drug for treating AIDS).
2)Generally, Gag and Env proteins are employed as antigens for
detecting anti-HIV-1 antibody. However, by using this enzyme in
combination as an antigen, the detection will be more sensitive.
3)Reverse transcriptases are used in the first step of RT-PCR reaction
for converting RNA to DNA. The HIV-1 reverse transcriptase can also
be applied for RT-PCR method.
4)Standards for SDS-PAGE (Fig.1), Western blotting (Fig.2), Dot
blotting, ELISA

Definition of activity: Activity of intake of 1 nmole of dTMP in 10
min at 37℃ is considered as 1 unit using poly(rA) and oligo(dT) as
template and primer.
Conditions of measurement: 50 mM Tris-HCl (pH 8.3), 10 mM MgCl2, 50mM
KCl, 3 mM DTT, 0.1% Nonidet P-40, 20 ug/ml poly(rA)・oligo(dT)12-18,
0.5 mM dTTP ([3H]dTTP ,~1 x 105 cpm), and 10-50 units/ml reverse
transcriptase.

Activity: ~5,000 units/ml

Purity: Over 90% by SDS-PAGE (CBB staining)

Form: 0.5 mg/ml in 50% glycerol, 40 mM Tris-HCl (pH8.3), 50 mM NaCl, 5
mM MgCl2, 0.1% Triton X-100, 1 mM DTT
 
 
Code
Quantity
                  Price
 
05-001
200 units
330 USD
05-002
1000 units
1,320 USD

Product
 
Information
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.23 mg/ml as measured by BCA method
Form: 50% glycerol 20mM Tris-HCl (pH7.5) 50mM NaCl 10mM mercaptoethanol
Storage: -20℃
 
 
Code
Quantity
                  Price
 
05-003
20 ug
290 USD
05-004
100 ug
990 USD

Product
 
Information
Full length recombinant HIV-1 type B Gag p24 protein expressed and proteolytically processed in E. coli in a soluble form without any tag attached.

Applications.
1. ELISA
2.Western blot as antigen
3. SDS-PAGE

Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 1 mg/ml as measured by BCA method
Form: 50% glycerol 20mM Tris-HCl (pH7.5) 50mM NaCl 10mM mercaptoethanol
Storage: -20℃
 
 
Code
Quantity
                  Price
 
05-005
20 ug
290 USD
05-006
100 ug
990 USD

Product
 
Information
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.42 mg/ml as measured by BCA method
Form: 50% glycerol 20 mM Tris-HCl (pH 7.5) 50 mM NaCl 10 mM mercaptoethanol
Storage: -20℃
 
 
Code
Quantity
                  Price
 
05-007
20 ug
290 USD
05-008
100 ug
990 USD

Product
 
Information
05-009
05-010

Storage temperature : Ship with dry-ice or ice-pack and store at -20℃ (longer period-80℃)

Product: Recombinant full-size HIV-1 subtype 1、clone pNL4-3 (Ref 2) expressed in E. coli.

Applications
1) Substrate for the HIV-1 protease activity assay.
2) It can be used in detection of anti-HIV-1 Gag antibody in
Western blotting or ELISA. All the anti-HIV-1 Gag antibodies
such as anti-p17 antibody anti-p24 antibody and anti-p15
antibody can be measured at the same time.

Purity: Over 90% by SDS-PAGE (CBB staining)

Protein concentration: 0.5~1.0 mg/ml as determined by BCA method.

Form: 20% glycerol 20mM Tris-HCl (pH7.5) 50mM NaCl 10mM
mercaptoethanol

Background: HIV-1 Gag p55 is a precursor protein of several proteins that form the core structure of AIDS virus which are indispensable to their reproduction. This protein is digested by HIV-1 protease first into intermediate products p41 and p15. Then p41 is digested into matrix protein p17 and capsid protein p24. Protein p15 is further digested into nucleocapsid protein p7 and to p6 and p1 whose functions are unknown (1).
 
 
Code
Quantity
                  Price
 
05-009
20 ug
330 USD
05-010
100 ug
990 USD

Product
 
Information
Code:05-011

Storage: Ship with ice-pack and store at-20℃.

Product: Full-size recombinant HIV-1 Nef protein expressed in
E.coli. No tag is attached.

Applications:
1) Functional studies of HIV-1 Nef protein
2) SDS-PAGE and Western blotting
3) ELISA

Purity: Over 90% by SDS-PAGE

Form: 50% glycerol 20 mM Tris-HCl (pH 7.5) 50 mM NaCl 10 mM
mercaptoethanol

Data Link: ProtKB: P03406 (NEF_HV1BR) GenBank: AAA44988.1
 
 
Code
Quantity
                  Price
 
05-011
20 ug
290 USD
05-012
100 ug
990 USD

Virology > HIV
Product
 
Information
Product: Polyclonal guinea pig antiserum raised against full-size recombinant HIV-1 reverse transcriptase
Applications: Western blotting. Immunoprecipitation. ELISA.IHC
Form: Undiluted antiserum added with 0.09% sodium azide .
Storage: Shipped at 4℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
65-003
100 ul
345 USD

Virology > HIV
Product
 
Information
Product: Polyclonal rabbit antiserum raised against intact recominant HIV-1 gag p24
Applications: Western blotting (X1000-3000) Immunoprecipitation. ELISA. IHC
Reactivity: Reactive with various substrains of HIV-1 p24 with little background noise. Reactive also with Gag precursor p55.
Form: Undiluted serum added with 0.09% sodium azide Storage:
Storage: Shipped at 4℃ or -20℃ and store at -20℃
Related Product: Recombinant HIV-1 Gag p24 (wothout tag)(#05-005. 006)
anti-HIV-1 gag p24 antibody (guinea pig serum) (#65-006)

Purified anti-p24 IgG solution without sodium azide is also available upon request
 
 
Code
Quantity
                  Price
 
65-004
100 ul
345 USD

Virology > HIV
Product
 
Information
Product: Polyclonal guinea pig antiserum raised against recombinant HIV-1 Gag p24 (no tag)
Applications: Western blotting (X1000-3000). Immunoprecipitation. ELISA.IHC
Reactivity: Reactive with various subtypes of HIV-1. Reactive also with Gag precursor p55
Form: Undiluted antiserum added with0.09% sodium azide
Storage: Shipped at 4℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
65-006
100 ul
345 USD

Virology > HIV
Product
 
Information
Code:65-008

Product:Polyclonal rabbit antiserum raised against full-size recombinant p17 of HIV-1 (no tag)
Applications: Western blotting (X1000-3000). Immunoprecipitation. ELISA. IHC
Reactivity: Reacts with p17 as well as the gag precursors such as p55
Form: Undiluted serum added with0.09% sodium azide.
Storage: Shipped at 4℃ or at-20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
65-008
100 ul
345 USD

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