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Code:02-300

Shipping and Storage : Sent at 4℃ or -20℃ and store at -20℃. Do not
freeze below -20℃

Product: Functional full-length recombinant λProtein Phosphatase (λ-
PPase) expressed in E.coli. It is a Mn+2-dependent protein
phosphatase with activity towards phosphorylated serine, threonine,
tyrosine and histidine residues. It is the 221 amino-acid product of
ORF221 open reading frame on bacteriophage lambda . This
product is an intact enzyme of high quality without tag.

Applications: λ-PPase can be used to release phosphate groups from
phosphorylated serine, threonine, tyrosine and histidine residues in
proteins. It should be noted that different proteins are
dephosphorylated at different rates. Optimal reaction temperature is
30℃. Inclusion of protease inhibitor cocktail and shortest
incubation time is desired when assays are done with crude samples.

Form: 400 U/ul λ-PPase in 50mM HEPES (pH 7.5), 100mM NaCl, 2mM
dithiothreitol,0.1 mM MnCl2, 0.1mM EDTA, 50% glycerol, 0.01% Brij
35.

Activity: 400 U/ul, where one unit is defined as the amount of enzyme
that hydrolyzes 1 nmole of p-nitrophenyl phosphate per minute at
30℃. Unit definition assays are performed with 50mM p-nitrophenyl
phosphate in λ-PPase buffer, supplemented with 2 mM MnCl2 in a 50 ul
reaction.

Specific Activity: ~400,000 U/mg

Purity: Greater than 95% as determined by SDS-PAGE (CBB staining)
that contains no detectable protease activity

Reagents Supplied with Enzyme:
10 x λ-PPase Reaction Buffer: [500mM Tris-HCl (pH 7.6), 1M NaCl, 20mM
dithiothreitol, 1mM EDTA, 0.1% Brij 35]
10 x Mn2+ (20 mM MnCl2)
 
 
Code
Quantity
                  Price
 
02-300
20000 U
110 USD
02-300-5
5x 20000 U
440 USD

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