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Molecular Genetics > Transcription・Translation
Product
 
Information
01-005 01-006

Backgrounds: LexA protein is the repressor of SOS regulon and binds specific DNA sequence called SOS box. It is used as a bait in yeast two-hybrid system.

Appications.
1. As an antigen of a control of bait protein expressionin western
blotting with anti-LexA antibody.
2. For the studies of SOS regulon.

Purity: Over 90% by SDS-PAGE (CBB staining)

Protein concentration: 0.8 mg/ml as measured by BCA method

Form: 50% glycerol 10 mM Tris-HCl (pH 7.5) 2 mM EDTA 100 mM NaCl 5 mM
mercaptoethanol

 
 
Code
Quantity
                  Price
 
01-005
20 ug
420 USD
01-006
100 ug
1,260 USD

Molecular Genetics > Transcription・Translation
Product
 
Information
62-009

Storage temperature: Ship at 4℃and store at -20℃

Reactivity: S. cerevisiae Sua7 / TFIIB protein

Immunogen: Rcombinant His-tagged full-size Sua7 protein

Applications
1.Western blotting. (1/1000~1/5000)
2.Immunoprecipitation
3.Chromatin Immuno-Precipitation
4.ELISA

Form: 0.1% sodium azide added to the antiserum

Background: The fundamental transcription factor TFIIB has the characteristics of stabilizing the DNA binding of TATA box-binding protein (TBP) and binding directly to DNA by its conformational change. Also its N terminal region binds to the RNA channel of RNA polymerase undertaking a very important role in the determination of transcription initiation point and promoter clearance. Sua7p is the TFIIB of budding yeast and is composed of 346 amino acid residues (aa)
 
 
Code
Quantity
                  Price
 
62-009
100 ul
345 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:63-101

Shipping and Storage : Ship at 4℃and upon arrival, aliquot and store at -20℃.

Reactivity: Swi6 protein of S. pombe

Immunogen: Recombinant full length protein corresponding to
Schizosaccharomyces pombe Swi6 aa 1-328

Applications  For the studies of RNAi mechanism
1. Western blotting (x 2,000~10,000 dilution) (Figure) Backgroud noise
was reduced with diluted antibody
2. Immunoprecipitation
3. Immunoflorescent staining
4.Chromatin Immuno-Precipitation

Form: Undiluted antiserum added with0.09% sodium azide
 
 
Code
Quantity
                  Price
 
63-101
50 ul
300 USD

Molecular Genetics > Transcription・Translation
Product
 
Information
Code:63-125 63-126

In the eukaryotic cells DNA is packaged repetitively into nucleosomes by means of interactions among two molecules of four classes of histone H2A H2B H3 and H4. Each of the histone proteins has an evolutionarily conserved amino-terminal ‘tail’ that protrudes from the nucleosome. This tail is the target of numerous diverse signaling pathways resulting in the addition of many post-translational modifications. These modifications include phosphorylation acetylation methylation ADP-ribosylation and mono-ubiquitination. Many important new modifications within the structured core and the carboxy-terminal tail regions of histones are also being identified. It is becoming increasingly clear that these modifications represent crucial regulatory events that govern the accessibility and function of the genome
Applications (see Ref 1)
1) Western blotting (1000 fold dilution). 2) Immunoprecipitation (CHIP assay).
Antigen: Synthetic peptide corresponding to the amino-terminal S. pombe histone H2B SAAEKKPASKAPAGKA
Reactivity: S. pombe histone H2B
Antibody: Undiluted rabbit antiserum added with 0.05 % sodium azide
Storage: -20℃ (long period -80℃)
Reference: This product has been used in the following reference.
1. Maruyama T. et al (Yanagida M). Histone H2B mutations in inner region affect ubiquitination centromere function silencing and chromosome segregation. EMBO J. 25: 2420-2431 (2006)
 
 
Code
Quantity
                  Price
 
63-125
50 μl
300 USD

Product
 
Information
code:10-201

Shipping and Storage: Ship with dry-ice and Store at -80℃

Product: Recombinant mouse Dnmt1 (aa 291-1620), His-Tag attached to N-
termial, expressed in baculovirus expression system.

Reagents Supplied with Enzyme 
Dnmt1 Reaction Buffer (5 x)
20mM S-adenosylmethionine (SAM) which was purified by
chromatography from the commercial reagent and dissolved in H2O
Note: SAM is very unstable. Store at -80℃

Applications
1)In vitro methylation of cytosine residues in hemimethylated
DNA at 5’….CG…3’.
2)Antigen for anti-mammalian Dnmt1 antibodies.

Form: 0.5mg protein/ml in 0.2M NaCl, 10mM HEPES (pH 7.4), 50% glycerol

Definition of specific activity: 1 unit is defined as the amount of
the enzyme that transfer 1 pmole of methyl group to poly dI-dC
substrate during 30 minutes at 37℃

Specific activity: 17 units/ul

Purity: Greater than 95% protein determined by SDS-PAGE (CBB staining)

Reaction Conditions
Incubate in 1 x Dnmt1 Reaction Buffer (20mM Tris-HCl, pH7.4, 0.5 mM
EDTA, 0.2 mM DTT, 5% glycerol) with 10μM S-adenosylmethionine (SAM)
at 37℃

 
 
Code
Quantity
                  Price
 
10-201
300 U
330 USD

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