DNA polymerase β (Rat) functional

Code:10-101

Backgrounds:DNA polymeraseβ is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized methylated deaminated etc.) (ref. 1).This product is highly purified full-length rat DNA polymeraseβ overproduced in E. coli with high enzymatic activity without any tag attached (ref.2).

Applications
1) For the studies on the mechanisms of base-excision repair of DNA damage
2) As a positive control of western blotting with anti-DNA polymeraseβ antibody

Properties of the product
Enzyme activity: 90 unit/ul (1unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37℃ in 60 min.)
Purity: Over 95% pure by SDS-PAGE analysis
Form: 1.3 mg/ml in 50mM Tris-HCl pH7.6 0.3M KCl 0.1mM EDTA 1mM DTT 20% glycerol

References: This product is described in ref. 2
1. Friedberg EC et al. DNA Repair and Mutagenesis 2nd ed. ASM Press (2006)
2. Date T. et al. Biochemistry 27: 2983 (1988)

Related product
#70-041 anti-DNA polymeraseβ (rat) antibody affinity purified cross-reacts with human and mouse homologs.

Anti-DNA polymeraseδ subunit 3/p66(PolD、human) antibody monoclonal (2A1C11)

Code:70-056

Applications:
1) Western blotting (1/2,000 dilution
2) Immunoprecipitation (Assay dependent)
Other applications have not been tested.

Antigen: Recombinant human p66 subunit of DNA polymerase delta

Reactivity: Human p66 protein. Not tested with other species

Isotype: IgG2B (kappa)

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS (pH 7.4), 50%
glycerol, sterile-filtered, azide free

Storage: Shipped at 4℃ or -20℃ and stored at -20℃.

Proliferating Cell Nuclear Antigen (PCNA) human functional

code:10-151、10-152

Storage: Sent at 4℃ or -20℃. Upon arrival spin-down and store at -20℃ (or at -80℃ for longer storage)

Source: Human PCNA was over-expressed in E. coli as a recombinant full-size protein without any tag and highly purified.

Applications confirmed:
1. Functional studies on DNA replication, recombination and repair. (Ref 2, 3, 5, 6, 7, 8, 9, 10).
2. Identification of proteins interacting with PCNA by using PCNA –conjugated resin. (Ref 1, 5)
3. Ubiquitination targets (Ref 4, 9, 10).
4 SDS-PAGE (Fig. 1). 5. Western blot (Fig. 2) . 6. Dot blot. 7. ELISA. Not tested for other applications.

anti-RRM2/RNR-R2 antibody rabbit polyclonal affinity-pure

70-050
Function: Ribonucleoside-diphosphate reductase subunit M2 (RRM2; 389 aa
45 kDa) also known as ribonucleotide reductase subunit R2 (RNR-R2) is a
rate-limiting subunit of an enzyme that catalyzes the formation of
deoxyribonucleotides from ribonucleotides. Deoxyribonucleotides in turn
are used in the synthesis of DNA.

Immunogen: Synthetic peptide (11 aa) in the C-terminal region of Human
and Mouse RRM2.

Application: WB IP IF IHC

Reactivity: human mouse rat hamster xenopus