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Information
Code:70-130

Reactivity: Human mouse and rat. Other species have not been tested.

Validation: Validated for Western Blotting by siRNA.
Immunogen: Purified GST-GRWD1 (human full-length) expressed in E. coli.
Applications:
1. Western blotting (1/1000~1/3000 dilution)
2. Immunoprecipitation (Assay dependent)
3. Chromatin Immuno-Precipitation (Assay dependent)
4. Immunofluorescence staining / Immunochemistry (1/100~1/1000
dilution)
 
 
Code
Quantity
                  Price
 
70-130
100 ug
345 USD

Product
 
Information
Code:70-133

Storage: Sent at 4℃ and upon arrival spin-down and store at -20℃

Reactivity: human mouse rat

Immunogen: Recombinant GST-human CDC6 (1-326 amino acids) expressed in E. coli

Validation: Specificity of reaction has been validated with siRNA in WB (Ref.5 & 7)

Applications:
1. Western blotting (1/1000~3000)
2. Immunoprecipitation (1/200)
3. Chromatin Immuno-Precipitation (assay dependent)
4. Immunofluorescence staining (1/200)

Form: 1 mg/ml in PBS with 50% glycerol. Filter-sterilized. Azide and carrier free

Function: CDC6 (Cell Division Control Protein 6 homolog) (human; 560 aa 62.7 kDa) is involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subcellular location: The protein is nuclear in G1 and cytoplasmic in S-phase cells.
Purification: The antiserum was first adsorbed with GST conjugated agarose column and
then the antibody in the pass-through fractions were purified with GST-CDC6 conjugated column.

Data base Link: uniprot/Q99741 (CDC6_HUMAN)
 
 
Code
Quantity
                  Price
 
70-133
100 ug
345 USD

Product
 
Information
Code:70-230

Key words: BAF complex Chromatin remodeling complex CREBBP CREST-BRG1 complex CYP27B1 gene c-Fos promoter Nuclear hormone receptor Neural stem cell Neuron NR2B promoter phospho-RB1-HDAC repressor complex Transcriptional coactivator Vitamin D receptor WINAC complex Zeb1

Applications
1) Western blotting (1/1000 dilution) (Fig. 1)
2) Immunoprecipitation (Assay dependent) (Fig. 2) 
3) Immunofluorescence staining (1/1000 dilution) (Fig. 3)
Not tested for other applications.
 
 
Code
Quantity
                  Price
 
70-230
100 ug
345 USD

Product
 
Information
Code:70-305

Storage: Shipped at 4℃, and upon arrival, spin-down and store at -20℃


Immunogen: Recombinant human Nup62 (aa 1-300) (GST-Nup62-His) 

Epitope: aa 1-179 (FG-repeat region)

Isotype: Rat IgG1 kappa

Product: Purified from serum-free culture medium of the hybridoma by proprietary chromatography under mild conditions.

Form: 1mg/ml in PBS, 50% glycerol, filter-sterilized.
Carrier protein and sodium azide.free.

Specificity: Specific to human (HeLa cells) and simian (Cos cells). The antibody did not react with mouse.

Applications
1. Western blotting (1/500 ~1/2000 dilution)
2. Immunoprecipitation (assay dependent)
3. Immunofluorescece / Immunocytochemistry (1/400)
4. ELISA (assay dependent)
5. When this antibody was micro-injected into the cytoplasm of the HeLa cells it accumulates into the nuclear pores as examined by immunofluorescence staining..




 
 
Code
Quantity
                  Price
 
70-305
200 ug
345 USD

Product
 
Information
Code:70-501

Shipping and Storage: Ship at 4℃and store at -20℃.

Reactivity: Human (HeLa, HEK293T, MCF7) and mouse (NIH3T3). Note that expression level of EDD varies greatly, depending on tissues and cell types (see Fig.1).

Immunogen: Synthetic peptide of human EDD protein corresponding to 394-408 amino acids, C-KWSESEPYRNAQNPS, conjugated with KLH

Applications
1) Western blotting (1/1,000)
2) Immunofluorescent staining 1/1,000)

Product: Affinity-purified from rabbit antiserum with immunogen peptide conjugated with agarose beads.

Form: 1 mg/ml in PBS (x1), 50% glycerol. Filter-sterilized. Azide and carrier free.

Reference: This antibody has been used in the following publications.
Watanabe S et al. GRWD1 regulates ribosomal protein L23 levels via the
ubiquitin-proteasome system. J Cell Sci. 2018 Aug 3;131(15). PMID:29991511 WB (human)
 
 
Code
Quantity
                  Price
 
70-501
100 ug
310 USD

Product
 
Information
code:70-657

SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. Unlike ubiquitination which targets proteins for degradation SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport gene expression cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. There are three confirmed SUMO isoforms in human; SUMO1 SUMO2 and SUMO3. SUMO2 and 3 show a high degree of similarity to each other and are distinct from SUMO1. Individual SUMO family members are all targeted to different proteins with diverse biological functions. SUMO2/3 forms poly-(SUMO) chains is conjugated to topoisomerase II and APP and regulates chromosomal segregation and cellular responses to environmental stress.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Molecular mass: SUMO2; proform 10871 Da with 95 aa (94-95 aa are removed from proform). SUMO3; proform 11637 Da with 103 aa (93-103 aa are removed from proform).
Applications:
1. Western blotting (1/1000)
2. Immunocytochemistry/Immunofluorescence staining (1/100~1/500)
3. Immunohistochemistry frozen section (1/100~1/500)
4. ELISA (Assay dependent concentration)
Other applications have not been tested.
 
 
Code
Quantity
                  Price
 
70-657
100 ug
190 USD

Product
 
Information
code:73-006

Key words: Angiotensin converting enzyme-like 3 Sperm Acrosomal cap IZUMO1 interacting protein
Function: ACE3 was identified as an IZUMO1 interacting protein from mouse sperm. ACE3 protein is exclusively localized in testis and sperm. However unlike the disruption of IZUMO1 or ACE elimination of ACE3 in mouse did not result in loss of sperm fertilizing ability.
Molecular mass: ACE3 consists of 737 amino acids with molecular mass of 85874 Da.
Applications:
1. Western blotting (1/500 dilution)
2. Immunoprecipitation (1/100 dilution)
3. Immunofluorescence staining (1/100 dilution)
Other applications have not been tested.
 
 
Code
Quantity
                  Price
 
73-006
100 ul
345 USD

Product
 
Information
code:73-018

Key words: Calreticulin CALR XCRP55 Calregulin Endoplasmic reticulum resident protein 60 (ERp60)
Lectin chaperon calcium homeostasis
Function: Calreticulin (CALR) is calcium-binding chaperone that promotes folding oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis
Molecular mass: CALR consists of 416 amino acids with molecular mass of 47995 Da.
Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100)
Other applications have not been tested.
 
 
Code
Quantity
                  Price
 
73-018
100 ul
220 USD

Product
 
Information
code:73-022

Key words: Calreticulin-3 CALR3 Calsperin EIF2 complex Spermatogenesis Differentiation Endoplasmic reticulum
Function: CALR3 capacity for calcium-binding may be absent or much lower than that of CALR. During spermatogenesis may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility.
Molecular mass: 44232 Da with 380 amino acids.
Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100 dilution)
3. Immunohistochemistry (1/100 dilution)
Other applications have not been tested.
 
 
Code
Quantity
                  Price
 
73-022
100 ul
345 USD

Product
 
Information
code:73-026

Key words: Canx Endoplasmic reticulum Chaperon Synaptic vesicle endocytosis Transmembrane Calcium ion binding

Function: Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse
Molecular mass: 67278 Da with 591 amino acids
Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100dilution).
Not tested for other applications.
 
 
Code
Quantity
                  Price
 
73-026
100 ul
345 USD

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