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Information
Code:70-112

POGZ (pogo transposable element-derived protein with zinc finger domain; 1410 aa 155 kDa) plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 (HP1 alpha) it plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.

Applications
1. Western Blotting (1/1000 dilution)
2. Immunofluorescece staining (1/500 dilution)
3. Immunoprecipitation (assay dependent)
Not tested for other applications
 
 
Code
Quantity
                  Price
 
70-112
100 ul
350 USD

Product
 
Information
Code:71-600

Shipping, Storega: Ship at 4℃ and store at -20℃

Reactivity; Human VRK1 protein. Not tested with other species.

Immunogen: Synthetic peptide corresponding to N-terminus of human VRK1,
MPRVKAAQAGRQSSAKRHL-C

Applications
1. Western blotting (1/200~1/1,000 dilution). Use of highly
sensitive chemiluminescence reagents such as Lumi-Light Plus (Roche) or
ImmunoStar○RLD (Wako, Tokyo) are recommended.
2. Immunoprecipitation (assay dependent)
3. Immunofluorescence staining (1/100 dilution)
4. Immunohistochemistry (assay dependent)
5. ELISA (assay dependent)

Product; Mouse monoclonal antibody (5D1) produced in serum-free medium
and purified by propriety chromatography under mild conditions (90~98%
pure).

Isotype; IgG1 kappa

Form; 1 mg/ml in PBS with 50% glycerol, filter-sterilized.

 
 
Code
Quantity
                  Price
 
71-600
100 ug
330 USD

Product
 
Information
70-115

Validation: Specificity was validated by western blotting with siRNA

Storage: Ship at 4℃ and aliquot and store at -20℃
Reactivity; Human and mouse rat hamster

Applications
1. Western blotting (1/200-1/2000 dilution).
2. Immunoprecipitation (assay dependent)
3. Immunofluorescence staining (1/100~1/1000 dilution)


Immunogen ; E. coli expressed and purified 6×His-tagged C-terminal 150 amino acids of human Cdt2
Form; Rabbit polyclonal antiserum added with 0.05 % sodium azide

Function; CDT2 (human 730 aa 79.5 kDa) is substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control DNA damage response and translesion DNA synthesis. The DCX(DTL) complex also named CRL4(CDT2) complex mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication.
 
 
Code
Quantity
                  Price
 
70-115
100 ul
350 USD

Product
 
Information
Code:51-004

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA ― for example to the number 5 carbon of the cytosine pyrimidine ring ― which involves reduction in gene expression. In adult somatic tissues DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
 
 
Code
Quantity
                  Price
 
51-004
50 ug
345 USD

Product
 
Information
Code:51-005

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA ― for example to the number 5 carbon of the cytosine pyrimidine ring ― which involves reduction in gene expression. In adult somatic tissues DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
 
 
Code
Quantity
                  Price
 
51-005
50 ug
345 USD

Product
 
Information
Code:70-345

Storage: Shipped at 4℃ or -20℃, and upon arrival, spin-down and store at -20℃.

Reactivity: Human, Tetrahymena, S. cerevisiae, and S. pombe.. Not tested in other species

Immunogen: Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664

Applications: See Table 1 for summary. For details read Ref.1
1) Clone 13C2 antibody is most suitable for Western blotting, in human, Tetrahymena ,S. cerevisiae, and S. pombe. Not tested in other species.
2) Clone 21A10 antibody is most suitable for Immunofluorescence staining / Immunocytochemistry in human, T.etrahymena, S. cerevisiae, and S. pombe. Not tested in other species.

Epitope determined: FGxxN for clone 13C2 and GLF for clone 21A10

Isotype: Mouse IgG1 (κ) for both clones.

Form: Protein A affinity-purified IgG, 1 mg/ml in PBS(-), 50% glycerol, filter-sterilized. Azide- and carrier- free
 
 
Code
Quantity
                  Price
 
70-345
100 ug
345 USD

Product
 
Information
Code:70-346

Storage: Shipped at 4℃ or -20℃, and upon arrival, spin-down and store at -20℃.

Reactivity: Human, Mouse, S. cerevisiae, S. pombe and Tetrahymena. Not tested in other species

Immunogen: Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664

Applications: See Table 1 for summary. For details read Ref.1
1) Western blotting, (S. cerevisiae, S. pombe,) Not suitable for human. Clone 13C2 is recommended for WB in human Tetrahymena and yeasts.
2) Most suitable for Immunofluorescence staining / Immunocytochemistry for human, mouse, Tetrahymena, S. cerevisiae, S. pombe.

Epitope determined: GLF (Ref.1)

Isotype: Mouse IgG1 (κ)

Form: Purified IgG, 1 mg/ml in PBS(-), 50% glycerol, filter-sterilized. Azide- and carrier- free
 
 
Code
Quantity
                  Price
 
70-346
100 ug
345 USD

Product
 
Information
Code:70-347

Storage: Shipped at 4℃ or -20℃, and upon arrival, spin-down and store at -20℃.

Reactivity: Human, Tetrahymena, S. cerevisiae, and S. pombe.. Not tested in other species

Immunogen: Synthetic peptides containing conserved N-terminal
sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664

Applications: See Table 1 for summary. For details read Ref.1
1) Clone 13C2 antibody is most suitable for Western blotting, in human, Tetrahymena ,S. cerevisiae, and S. pombe. Not tested in other species.
2) Clone 21A10 antibody is most suitable for Immunofluorescence staining / Immunocytochemistry in human, T.etrahymena, S. cerevisiae, and S. pombe. Not tested in other species.

Epitope determined: FGxxN for clone 13C2 and GLF for clone 21A10

Isotype: Mouse IgG1 (κ) for both clones.

Form: Protein A affinity-purified IgG, 1 mg/ml in PBS(-), 50% glycerol, filter-sterilized. Azide- and carrier- free
 
 
Code
Quantity
                  Price
 
70-347
50 + 50 ug
345 USD

Product
 
Information
Code:70-120

Shippiong, Storage: Ship at 4℃. Upon arrival, spin-down, aliquot and
store at -20℃.

Reactivity: Reacts with human mouse, rat and hamster. Not tested in
other species.

Immunogen: Purified His6-tagged human MCM7 protein encompassing 562 -719
amino acids.

Applications
1) Western blotting (1/1,000~1/5,000 dilution).
2) Immunoprecipitation (assay dependent)
3) Immunofluorescence staining (1/200~1/1,000 dilution).

Product: Purified IgG from the rabbit antiserum. 1 mg/ml in PBS, 50%
glycerol, filter-sterilized. Azide- and carrier-free.
 
 
Code
Quantity
                  Price
 
70-120
100 ug
330 USD

Product
 
Information
Code:70-130

Reactivity: Human mouse and rat. Other species have not been tested.

Validation: Validated for Western Blotting by siRNA.
Immunogen: Purified GST-GRWD1 (human full-length) expressed in E. coli.
Applications:
1. Western blotting (1/1000~1/3000 dilution)
2. Immunoprecipitation (Assay dependent)
3. Chromatin Immuno-Precipitation (Assay dependent)
4. Immunofluorescence staining / Immunochemistry (1/100~1/1000
dilution)
 
 
Code
Quantity
                  Price
 
70-130
100 ug
345 USD

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