Registration
          
Cart
      
Infomation of Product
Sort
All topics:45 topics
21 topic~30 topics
Product
 
Information
Code:65-063

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. The primary function of NS5a is not known but from the comparative studies with other viruses it is predicted to play a role in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. FACS

Immunogen: A region of NS5a protein of HCV genotype 1b (ref.4) expressed in E. coli

Conjugate: Biotin conjugated [biotin] / [IgG] = 9.6

Isotype: Mouse IgG 2a kappa

Form: 0.8 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5a protein of genotype 1b. Not tested in other genotypes.
 
 
Code
Quantity
                  Price
 
65-063
50 ug
315 USD

Product
 
Information
Code:65-064

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. The primary function of NS5a is not known but from the comparative studies with other viruses it is predicted to play a role in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. FACS

Immunogen: A region of NS5a protein of HCV genotype 1b expressed in E. coli

Conjugate: FITC conjugated [FITC] / [IgG] = 4.8

Isotype: Mouse IgG 2a kappa

Form: Purified monoclonal antibody (IgG) 1.4 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5a protein of genotype 1b. Not tested in other genotypes.
 
 
Code
Quantity
                  Price
 
65-064
50 ug
315 USD

Product
 
Information
Code:65-068

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of hepatitis. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS5b protein of HCV genotype 1b expressed in E. coli

Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

Isotype: Mouse IgG2b kappa

Form: 0.7 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5b protein of genotype 1b. Not tested in other genotypes.
 
 
Code
Quantity
                  Price
 
65-068
50 ug
315 USD

Product
 
Information
Code:65-069

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The polyprotein encoded is processed by host cell and viral proteases. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS5b protein (the nucleotide sequence is shown in ref.4) of HCV genotype 1b expressed in E. coli

Conjugate: FITC conjugated [FITC] / [IgG] = 6.0

Isotype: Mouse IgG2b kappa

Form: 1.3 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5b protein of genotype 1b. Not tested in other genotypes.
 
 
Code
Quantity
                  Price
 
65-069
50 ug
310 USD

Product
 
Information
Code:65-021

HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). p24 is used as a marker antigen for observing the patient’s condition after treatment as it indicates the amount of virus in the blood.
The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus subtype B (2) and was highly purified by several steps of chromatography (3 4).
Using this antiserum in Western blotting the bands of 24 kD 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells (Fig. 1).
This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

Applications
1. Western blotting 2. Immunoprecipitaiton 3. Immunofluorescence  staining 4. ELISA          Not tested in other application

Form: Biotin conjugated IgG (0.9 mg/ml) in PBS 50% glycerol filter-sterilized
Storage: Sent at 4℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
65-021
100 ug
420 USD

Product
 
Information
65-110

Storage and shipping temperature: Ship at 4℃ and store at -20℃.

Specificity: Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2 H3N2 and avian H5N1 H5N2 and H1N1 (seasonal pandemic and swine). No cross reactivity with influenza B viruses.

Immunogen: Human Influenza A Virus (H2N2) Okada strain

Applications
1) Western blotting (300~1000 fold dilution)
2) Immuno-precipitation (100 fold dilution)
3) Immunofluorescent staining (200 fold dilution)
4) Immunohistochemistry (200 fold dilution)
5) ELISA (assay dependent)

Isotype: mouse IgG2A


Form: 1 mg/ml in PBS 50% glycerol filter sterilized. No additive
nor carrier protein added
 
 
Code
Quantity
                  Price
 
65-110
100ug
345 USD

Product
 
Information
Code:65-200

Shipment andStorage: Ship at 4℃ and store at -20℃. Do not freeze below -20℃

Reactivity: Reacts with gQ1 of HHV6A and HHV6B. However, this antibody is not recommended for IP of HHV-6B due to conformation specificity.

Immunogen: His6-tagged recombinant gQ1 of HHV-6A encoding 3-422 amino acids expressed in E. coli.

Applications
1) Western blotting (1/500~1/1,000 dilution)
2) Immunoprecipitation (assay dependent)
2) Imunofluorescence staining and Immunocytochemistry (1/100~1/3,200 dilution)
4) Flow Cytometry (1/100)
5) ELISA (assay dependent)

Isotype: mouse IgG1 kappa

Product: Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE.

Form: 1 mg/ml in PBS, 50% glycerol, filter sterilized. Azide- and carrier-free.
 
 
Code
Quantity
                  Price
 
65-200
50 ug
345 USD

Product
 
Information
Code:65-111

Storage temperature: Ship at 4℃ and store at -20℃. Avoid repeating freeze-thaw.

Specificity: Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34). No cross reactivity with influenza B viruses.

Immunogen: Human Influenza A Virus H3N2

Applications
1)Western blotting (300~1,000 fold dilution)
2)Immunohistochemistry (200 fold dilution)
3)ELISA (assay dependent)

Isotype: mouse IgG2A


Form: 1 mg/ml in PBS, 50% glycerol, filter sterilized. No additive nor carrier protein added
 
 
Code
Quantity
                  Price
 
65-111
50 ug
345 USD

Product
 
Information
Code:65-155

Background: Matrix protein 1 (M1) plays critical roles in virus replication from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1 a non-raft-associated protein into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion where it binds the RNP. During virus entry into cell the M2 ion channel acidifies the internal virion core inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus where viral transcription and replication can take place.
M1 of Influenza B virus consists of 248 amino acids with molecular mass of 27415.
 
 
Code
Quantity
                  Price
 
65-155
100 ug
345 USD

Product
 
Information
Code:65-160

Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2 releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
Post-translational modification
HAo is consists of 584 amino acids with molecular mass of 63275. In natural infection inactive HA is matured into HA1 and HA2. By sequence similarity it is indicated to be palmitoylated.
 
 
Code
Quantity
                  Price
 
65-160
100 ug
345 USD

About Security  |   Policy  |   Escape  |   Site map  |   Address
Copyright(C) BioAcademia, Inc. 2006. All Rights Reserved.