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All topics:33 topics
21 topic~30 topics
Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-503

Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor HB-EGF (1 2). The former functions as juxtacrine and the latter as paracrine growth factor. HB-EGF activates EGFR and ErbB4 and promotes the development of many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin (3). Non-toxic mutant of diphtheria toxin CRM197 inhibit HB-EGF function which is elevated in most ovarian cancer is being tested as an anticancer drug (4).

Applications
1)Western blotting (0.2~1 ug /ml) 2) Immunoprecipitation.
3)Indirect immuno-fluorescence staining
Properties of the product
Antigen: Recombinant human HB-EGF ectodomain expressed in SF21 cell.
Antibody: Produced in serum-free medium and purified by combination of chromatography
Isotype: IgG1(mouse) Epitope: EGF domain Reactivity: React with human but not with mouse
Form: Biotinylated IgG (biotin/IgG = 7.5) 0.6 mg/ml in PBS 50% glycerol filter-sterilized azide-free

Reference:
1. Higashiyama S. et al. Science 251: 936 (1991)
2. Prenzel N. et al. Nature 402: 884 (1999)
3. Iwamoto R. et al. EMBO J 13: 2322 (1994)
4. Miyamoto S. et al. Cancer Res. 64:5720 (2004)

Related product: # 71-501 Anti-HB-EGF (human) antibody monoclonal (4G10). # 01-515 Diphtheria toxin mutant CRM197. # 01-517 Diphtheria toxin..
 
 
Code
Quantity
                  Price
 
71-503
50 ug
330 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-185

Storage: Ship at 4℃and store at -20℃. Do not freeze.

Specificity: Reacts with human EID1 protein. Not tested for other
species.

Immunogen: Synthetic peptide containing amino acids 1-19 of human EID1
protein

Applications
1) Western blotting (~1 ug/ml).
2) Immunofluorescence staining (~1 ug/ml).
Other applications have not been tested.

Isotype: Mouse IgG2a (kappa)

Form: 1mg/ml in PBS (pH 7.4), 50% glycerol, sterilized by filtration.
Azide- and carrier-free.
 
 
Code
Quantity
                  Price
 
71-185
100 ug
345 USD

Product
 
Information
Code:63-154

Shipping and Storage: Ship at 4 ℃ and store at -20℃

Applications
1) Western blotting (1,000 fold dilution)
2) Immunofluoresece staining (100 fold dilution)

Antibody: Recombinant Shugosin 1 was used as immunogen for rabbit immunization
and affinitypurifivation.

Form: 0.2 mg/ml affinity purified anti-Shugosin 1 antibody in 1 mg/ml BSA, PBS (pH 7.2), 50% glycerol, filter-sterilized, azide free

 
 
Code
Quantity
                  Price
 
63-154
100 ul
350 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-175

Storage: Ship at 4℃ and store at -20℃

Reactivity: Human and mouse RBP2. Can detect endogenous levels of
RBP2.

Applications
1. Western blotting (~1 μg/ml) Other applications were not
tested.

Immunogen: A synthetic peptide corresponding to human RBP2, amino
acids 1416-1434.

Isotype: Mouse IgG2a kappa

Purification: IgG fraction is purified from supernatant of the
hybridoma cell culture in serum-free medium

Form: 1mg/ml in PBS, 50% glycerol, filter-sterilized
 
 
Code
Quantity
                  Price
 
71-175
100 ug
345 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-177

Storage: Shipt at 4℃ or at -20℃ and store at -20℃

Reactivity: Human and mouse RBP2. Can detect endogenous levels of RBP2.

Immunogen: A synthetic peptide corresponding to human RBP2, amino acids 1416-1434.

Applications
1. Western blotting (~1ug/ml)
2. Immunofluorescence staining
3. Flow Cytometry (1µg for 106 cells.)

Isotype: Mouse IgG2a kappa

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol,
filter-sterilized

Backgroud:RBP2 was originally identified as a retinoblastoma binding
protein. It is also known as JARID1A (Jumonji, AT rich interactive
domain 1A). RBP2 plays both negative and positive roles in RB-mediated
transcriptional activation, depending on the kinds of genes and
regulates differentiation by its function as an H3K4 histone demethylase
 
 
Code
Quantity
                  Price
 
71-177
100 ug
345 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:70-203

Dnmt1 (DNA (cytosine-5-)-methyltransferase 1) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics). Hypermethylation in promoter regions is asssociated with repression of gene expression. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1 2). This antibody was raised by Prof. S. Tajima of Osaka Univ. (3 4)
Applications
1) Western blotting (0.2~1 ug /ml)
2) Immunoprecipitation. Especially good for denatured protein and useful for ChiP assays
3) Indirect immuno-fluorescence staining
Properties of the product

Antigen: Recombinant mouse Dnmt1 (amino acids 1037-1386) purified by SDS-PAGE .

Antibody: Affinity-purified with the recombinant Dnmt1(1037-1386)

Reactivity: mouse human xenopus

Form: 1 mg/ml in PBS 50% glycerol 0.05% sodium azide (and trace of ammonium sulfate)

Storage: -20℃ (long period; -80℃)

 
 
Code
Quantity
                  Price
 
70-203
50 ug
290 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
Code:71-190

EID1 (EP300 interacting inhibitor of differentiation 1) encodes a 21 kDa protein which interacts with RB1 and EP300 and acts as a repressor of MYOD1 transcription. EID1 inhibits EP300 and CBP histone acetyltransferase activity. It may be involved in coupling cell cycle exit to the transcription of genes required for cellular differentiation.
 
 
Code
Quantity
                  Price
 
71-190
100 ug
345 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
code:70-206

anti- Dnmt3b (DNA (cytosine-5-)-methyltransferase 3b)KO-Validated

Applications
1)Western blotting (1000-fold dilution)
2)Immunoprecipitation (1000-fold dilution)
3) ChIP assays
3)Indirect immuno-fluorescence staining (1000-fold dilution)
4) Immunohistochemistry (1000-fold)

Immunogen: Recombinant mouse Dnmt3b (amino acids 1-181)

Antibody: Affinity-purified with the recombinant Dnmt3b

Reactivity: Mouse

Form: ~0.3 mg/ml in PBS 50% glycerol 0.05% sodium azide (and trace of ammonium sulfate)

Storage: -20℃ Do nmot freeze.
 
 
Code
Quantity
                  Price
 
70-206
100 ul
350 USD

Product
 
Information
code:03-001、03-001-5

Shipping and Storage::Ship at 4℃ and store at -20℃

Product: Recombinant mature human EGF protein expressed in E. coli. Aa
971-1023 of Pro-EGF

Applications
1.EGF is commonly used as a supplement in serum-free or reduced serum
media for culture of mammalian cells.
2.Studies of the human EGF receptor, transmembrane signaling and
protein phosphorylation
3.Western blotting control for anti-EGF antibodies
4. Acceleration of the healing process of epidermis damage on skin,
cornea, etc.
5.Widely used in cosmetics, such as whitening, anti-wrinkle, anti-
aging, etc.

Activity: The ED50 as determined by a cell proliferation assay using
MTS assay kit (CellTiter 96 AQueous Assay, Promega) with NHEK cells
(normal human epidermal keratinocytes) was < 0.1 ng/ml, corresponding
to a specific activity of < 1 x 107 units/mg.

Purity: >95% as determined by SDS-PAGE (CBB staining)

Form: 1.0 mg/ml in 10mM Na-phosphate (pH7.2), 50% glycerol, filter-
sterilized
 
 
Code
Quantity
                  Price
 
03-001
50 ug
125 USD
03-001-5
5 x 50 ug
375 USD

Product
 
Information
code:03-003、03-003-5

FGF-1 (acidic FGF) is a member of the fibroblast growth factor (FGF) family which binds heparin. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development cell growth morphogenesis tissue repair tumor growth and invasion (ref.).

Applications
1. Use as a supplement in serum-free or reduced serum media for culture of mammalian cells.
2. Studies of the human FGF1 receptor transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF antibodies
4. Widely used in cosmetics such as whitening anti-crinkle anti-aging etc.
Product: Full length recombinant mature FGF-1 (15.8 kDa 140 amino acids) expressed in E. coli.
Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96
Promega) with Balb/c3T3 cells was < 10 pg/ml corresponding to a specific activity of < 1 x 108 units/mg.
Purity: >98% as determined by SDS-PAGE (CBB staining)
Form: 2.0 mg/ml in PBS (10mM Na-phosphate 150mM NaCl) pH7.2 50% glycerol filter-sterilyzed
Storage: -20℃ (for long-term storage -80℃)
 
 
Code
Quantity
                  Price
 
03-003
50 ug
260 USD
03-003-5
5 x 50 ug
780 USD

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