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All topics:182 topics
11 topic~20 topics
Product
 
Information
Code:71-171

Storage: Ship at 4℃ or -20℃ and store at -20℃. Do not freeze below -20℃.

Specific reactivity: Specific to human Rb phosphorylated at Ser795. Not tested for other species. Can detect endogenous levels of Rb phosphorylated at Ser795 in most cell lines.

Immunogen: A synthetic peptide containing phospho-Ser795 of human Rb

Applications
1. Western blotting (~1ug/ml)  
2. ELISA Other applications were not tested.

Isotype: Mouse IgG2a ()

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized

 
 
Code
Quantity
                  Price
 
71-171
100 ug
345 USD

Product
 
Information
Code:71-173

Storage: ship at 4℃ or -20℃and store at -20. Do not freeze by storing
below -20℃.

Specific Reactivity: Specific to human Rb phosphorylated at Thr821. Not
tested for other species. Can detect endogenous levels of Rb
phosphorylated at Thr821 in most cell lines.

Immunogen: A synthetic peptide containing phospho-Thr821 of human Rb

Applications
1. Western blotting (~1ug/ml)    
2. ELISA    Other applications were not tested.

Isotype: Mouse IgG1(kappa)
Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized
 
 
Code
Quantity
                  Price
 
71-173
100 ug
345 USD

Antibodies > Molecular Genetics
Product
 
Information
Code:63-105

Cnd2 protein is a regulatory subunit of the condensin complex which is required for conversion of interphase chromatin into mitotic condensed chromosomes in S. pombe. The condensin complex also plays a role during interphase in processes of DNA repair and recombination.

Applications
1.Western blotting (500 fold dilution) (Ref.123)
2.Immunofluorescent staining (Ref. 3)
Not tested for other applications
Immunogen: Recombinant GST-Cnd2 fusion protein expressed in E. coli
specificity: Reacts with S. pombe Cnd2 protein. Not tested for
other species
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: shipped at -20℃ and stored at -20℃
References: This antibody was used in 3 references by Yanagida M group.
 
 
Code
Quantity
                  Price
 
63-105
100 ul
400 USD

Product
 
Information
code:63-107

Cut5/Rad4/Dre3 protein is an essential component for DNA replication and also for the damage and checkpoint control which couples S and M phases (1 2). It interacts with chromatin proteins to form the complex required for the initiation and progression DNA synthesis. It contains 4 BRCT domains and the molecular mass is 74.1 kDa with 648 amino acids.
Applications
1. Western blotting (500 fold dilution) 2. Not tested for other applications
Immunogen: Recombinant GST-fused with the N-terminal half of Cut5 protein.
specificity: Reacts with S. pombe Cut5/Rad4 protein. Not tested for other species.
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: -20 ℃ (long period -70℃)
References: This antibody was used in the following references.
1. Saka Y. et al. Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2 a protein with BRCT motif with Cut5 and Chk1.Genes Dev.11:3387 (1997)
2. Saka Y. et al. Fission yeast cut5 links nuclear chromatin and M phase regulation in the replication checkpoint control. EMBO J. 13:5319 (1994)
 
 
Code
Quantity
                  Price
 
63-107
100 ul
350 USD

Product
 
Information
Code:63-113

Cut15 protein is an essential component for DNA replication and also for the damage and checkpoint control which couples S and M phases (1 2). It interacts with importin alpha (Imp1p) which together functions in nucleocytoplasmic transport and cell cycle progression . It contains 4 BRCT domains and the molecular mass is 60.4 kDa with 542 amino acids.

Applications
1. Western blotting (100-1000 fold dilution)
Not tested for other applications

Immunogen: Recombinant GST-fusion protein with the full length of Cut15 protein

Specificity: Reacts with S. pombe Cut15 protein. Not tested for other species

Form: Rabbit antiserum added with 0.05 % sodium azide

Storage: Shipped at 4 ℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
63-113
100 ul
350 USD

Product
 
Information
Code:63-119

Schizosaccharomyces pombe Dis2 is a serine/threonine protein phosphatase which is highly similar to mammalian type 1 phosphatase (PP1). Protein phosphatases are known to play critical roles in cell cycle regulation in fission yeast. Fission yeast has two type 1 protein phosphatases Dis 2 and Sds21. They are 37 kDa proteins and their amino acid sequences are 80% identical to each other and to mammalian PP1 homologs. Dis 2 and Sds21 are necessary for mitotic chromosome disjunction and have overlapping functions. Their disruptants are lethal only when both genes are disrupted. Dis 2 is known to be enriched in nuclei.
1. Immunoblotting (dilution: 1/300~1/1000)
2. Immunoprecipitation
 
 
Code
Quantity
                  Price
 
63-119
100 ul
350 USD

Product
 
Information
Code: 63-121

Shipping and Storage: Shipp at 4℃ and upon arrival, centrifuge briefly, aliquot and store at -20℃ .

Specificity: Reacts with S. pombe Dis2 protein phosphorylated at Thr316.

Immunogen: Synthetic peptide NWHMT(PO3)PPRKN conjugated to KLF

Applications
Western blotting (1/1,000~1/2,000 dilution). Not tested for other
applications

Form: Rabbit antiserum added with 0.05 % sodium azide
 
 
Code
Quantity
                  Price
 
63-121
50 ul
290 USD

Product
 
Information
code:63-123

S. pombe Dis3 protein is an essential component for mitotic segregation (ref.1). It is a component of the exosome 3->5 exoribonuclease complex. It is required for the 3-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran and has a 3-5 exonuclease activity. It is composed of 970 amino acids with molecular mass of 110 kDa. It is highly conserved functionally and structurally from yeast to human.
 
 
Code
Quantity
                  Price
 
63-123
100 ul
300 USD

Product
 
Information
Code:63-133

Storage: Shipped at 4℃or -20℃ and stored at -20℃
Reactivity: S. pombe
Immunogen: Recombinant S. pombe full-length Pad1

Applications:
1. Immunoblotting (dilution: 1/300~1/1000)
2. Immunoprecipitation
Form: Rabbit antiserum added with 0.05 % sodium azide

Background:Schizosaccharomyces pombe Pad1 a 35 kDa protein is a component of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Transcription factor Pap1 is controlled by the functional interaction between the positive regulator Pad1 and negative regulator Crm1. Both proteins are essential for cell viability and for the maintenance of chromosome structure. Pad1 is also responsible for resistance to staurosporine and other drugs such as cycloheximide and caffeine.
 
 
Code
Quantity
                  Price
 
63-133
100 ul
350 USD

Product
 
Information
Code:63-135

Schizosaccharomyces pombe Ppa2 is a type 2A-like serine/threonine-protein phosphatase catalytic subunit whose polypeptide sequence has ~80% identity to those of mammalian type 2A phosphatases. Ppa2 determines the sensitivity to okadaic acid which is an inhibitor of protein serine/threonine phosphatases. The loss of the ppa2 gene causes cells to be hypersensitive to the okadaic acid. Ppa2 plays important roles in cell cycle control. It may be involved in controlling the entry into mitosis possibly acting as an inhibitor (ref.1). Ppa2 is abundant in the cytoplasm in contrast to the type 1-like phosphatase Dis2 which is enriched in the nucleus. Thus Ppa2 may perform major functions outside the nucleus.
 
 
Code
Quantity
                  Price
 
63-135
100 ul
350 USD

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