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Immunology > Immunoregulation
Product
 
Information
01-525

Cholera toxin is a complex consisting of one molecule of A-subunit (27.2 kD) and 5 molecules of B-subunit (11.6 kD). It adsorbs to GM1 ganglioside on the surface of target cells by the B subunit

Applications
1. Adjuvant for mucosal immunity. Stimulate B-lymphocytes. Vaccine
development
2. Tract tracing in neurological research taking advantage of GM1
ganglioside binding and retrograde transport.

Form:1 mg/ml in 20 mM Tris-HCl pH 6.8、0.2M NaCl 10% glycerol Filter-sterilized







 
 
Code
Quantity
                  Price
 
01-525
50 ug
310 USD

Molecular Biology / Cell Biology > Signal Transduction
Product
 
Information
01-531

Storage: Ship at 4℃ or -20℃, and store at -20℃ (For longer period,  years, store at -80℃)

Product: Recombinant streptolysin O. Functional in membrane pore   formation to introduce molecules into living animal cells. The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci (His6-tagged to the signal peptide removed N-terminal of SLO). The specific activity is as high as 1,200,000- 2,000,000 hemolytic units (HU) /mg (depending on Lot). 

Applications
1) Functional studies
2).Reagent for membrane pore formation to introduce small-to-   macromolecules into living cells (For protocol refer to or other references; Walev I et al “Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.” PNAS 98: 3185-3190 (2001) PMID: 11248053)

3).Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent), ELISA
4) Western blotting, Dot blotting,
5) Immuno-chromatography
6) SDS-PAGE

Measurement of the activity: Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37℃ for 30 min.

Purity: Over 98% by SDS-PAGE (see Fig.1)

Form: 1 mg/ml in PBS (-), 1 mM DTT, 50% glycerol, sterilized by filtration.
 
 
Code
Quantity
                  Price
 
01-531
20 ug
290 USD
01-531-5
5 x 20 ug
870 USD

Bacteriology / Bacterial Toxin Proteins > Bacterial Toxins
Product
 
Information
Code:64-001

Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. In the hemolytic streptococci infection the antibodies to various proteins secreted from the bacteria such as toxins increase. For the diagnostic confirmation of SLO production by the infection the measurement of the antibody ASLO (Anti-Streptolysin O) is most widely used.

Applications
1) Western blotting (x2000~10000 dilution)
2) Neutralization of hemolysis by Streptolysin O (SLO)
3) Immunoprecipitaion
4) ELISA

*Most of the anti-Streptolysin O polyclonal antibodies (ASLO) sold in the current market were obtained by immunizing with SLO purified from the culture supernatant of hemolytic Streptococci. But the SLO preparations were contaminated with other secreted proteins such as NADase. This product is highly specific to SLO because of the purity of immunogen

Immunogen: His6-tagged full-size recombinant SLO of S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli

Reactivity: Streptolysin O of Group ACand G origins

Form: Undiluted serum added with 0.09 % sodium azide.

Storage: Shipped at 4℃ or -20℃ and store at -20℃
 
 
Code
Quantity
                  Price
 
64-001
100 ul
345 USD

Bacteriology / Bacterial Toxin Proteins > Bacterial Toxins
Product
 
Information
Code:64-005

NAD (nicotinamide adenine dinucleotide) hydrolyzing enzyme is one of the extracellular enzymes and toxins produced by hemolytic streptococci. Although its function as a toxin is largely unknown it has been suggested to be related to pathogenicity of acute infection (1). NADase is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and the antibodies cross-react each other. Upon infection of hemolytic streptococci the antibody titer to the NADase increases similarly to anti-SLO (Strreptolysin O) antibody.
Application
1) Western blotting (x 2000~10000 dilution)
2) Immunoprecipitation
3) Neutralization of NADase activity
4) ELISA
 
 
Code
Quantity
                  Price
 
64-005
100 ul
345 USD

Bacteriology / Bacterial Toxin Proteins > Bacterial Toxins
Product
 
Information
Code:64-010

Diphtheria toxin (535 aa 58 kDa) is an exotoxin secreted as a proenzyme by Corynebacterium diphtheriae the pathogen bacterium that causes diphtheria. The toxin gene is encoded by a bacteriophage. The proemzyme undergoes proteolytic nicking and reduction of disulfide bond giving rise to subunit A (21 kDa) and B (37 kDa). Subunit B (C-terminal) recognizes cellular receptor HB-EGF receptor and subunit A (N-terminal) catalyzes the ADP-ribosylation of eukaryotic elongation factor-2 (eEF2) inactivating this protein.
Applications:
1. Western blotting (1/2000 dilution).
2. ELISA (1/2000~1/7000 dilution. Could detect 1 ng or less of the toxin)
3. Immunoprecipitation (1/200~ 1/1000 dilution depending on assay)
4. Dot blotting (1/1000 dilution. Could detect 1 ng or less of the toxin)  
Immunogen: Purified nontoxic mutant Diphtheria toxin CRM 197 (Ref 1 Product #01-515)
 
 
Code
Quantity
                  Price
 
64-010
100ul
345 USD

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