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Molecular Genetics > DNA Recombination
Product
 
Information
62-101

Storage: Sent at 4℃ and store at -20℃

Reactivity: S.cervisiae Rad 51 protein. The specificity of reaction

Immunogen: Recombinant His-tagged Sc Rad51 protein (full-size)

Applications
1) Western blotting (1/1000~1/10000 dilution) (Fig. 1 2)
2) Immunoprecipitation.
3) Chromatin Immunoprecipitaiton
4) Immunoflorescence staining

Form: Whole antiserum added with 0.09 % sodium azide

Background: S. cerevisiae Rad 51 protein (400 aa 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. Dmcl Rad55 Rad57 are paralogs of Rad51 and they form complex with Rad51 and Rad52 in mediating recombination processes
 
 
Code
Quantity
                  Price
 
62-101
100 ul
345 USD

Product
 
Information
Code:10-003、10-004

Backgrounds:Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands (1). Rad52 functionally and physically interacts with Rad51 Dmc1 and RPA in recombination processes. In radiation damaged cell Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and forms nuclear foci

Product:Expressed as a tagged protein in E. coli and highly purified

Form: 1 mg/ml in 20 mM Tris-HCl pH8.0
   100 mM KCl 2 mM 2ME 0.5 mM EDTA 20% glycerol

Reference: This product was used in reference 3
1. Friedberg EC et al. DNA Repair and Mutagenesis 2nd ed. ASM Press
2. Kitao H & Yuan Z M J. Biol. Chem. 277:48944 (2002)
3. Kurumizaka H et al. J Biol Chem 276: 35201

Related Products:
70-011 anti-Rad52 (human) rabbit affinity purified antibody
10-001 Rad51 (human) protein
70-001 Anti-Rad51 (human) rabbit antiserum
 
 
Code
Quantity
                  Price
 
10-003
20 ug
420 USD
10-004
100 ug
1,260 USD

Molecular Genetics > DNA Recombination
Product
 
Information
Catalogue number: 70-015

Shipping and Storage: Ship at 4℃ and store at -20 ℃. Do not freeze.

Specific Reactivity: human, mouse, rat, hamster

Immunogen: Full-size recombinant human Rad51 protein produced in E.
coli.

Appliccations
1) Western blotting (1/1,000~1/3,000)
2) Immuno-precipitation (Asssay dependent)
3) Immunofluorescence staining (1/1,000)
4) ELISA (Assay dependent)

Form; 1 mg/ml PBS ( 20mM Na-phosphate pH7.5 , 0.15M NaCl), 50% glycerol

Purity: Affinity-purified as IgG with Protein A.
 
 
Code
Quantity
                  Price
 
70-015
50 ug
290 USD

Product
 
Information
Code:02-040、02-040-5

T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity (12). It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
Applications: 1. Promoting DNA replication and recombination by stabilizing single-stranded DNA (1)
2. Increase specificity and yields of long PCR products (3)
Quality Assurance: Greater than 95% of protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
Form: 10 mg/ml in 20mM Tris-HCl (pH 8.0) 100mM NaCl 0.5mM dithiothreitol 1mM EDTA 50% glycerol
Storage: -20℃
 
 
Code
Quantity
                  Price
 
02-040
200 ug
100 USD
02-040-5
5 x 200 ug
345 USD

Product
 
Information
Code: 02-042、02-042-5

Shipping and Storage: Ship at 4℃ or -20 C and store at -20℃. Do not
freeze.


Applications:
1.Functional single-stranded DNA-binding protein for studying DNA
replication and recombination
2.Enhancement of the specificity and yield of PCR

Background: E.coli single-stranded DNA binding protein (SSB) binds to
single-stranded DNA with high specificity (1, 2). It is involved in DNA
replication and recombination in vivo. The SSB gene was expressed as the
recombinant protein in E.coli highly purified. The molecular mass is
18.9 kDa.

Form: 2 mg/ml in 20 mM Tris-HCl (pH 7.6), 200 mM NaCl, 1 mM
dithiothreitol, 1mM EDTA, 50% glycerol

Purity: Greater than 95% purity as determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.


 
 
Code
Quantity
                  Price
 
02-042
200 ug
95 USD
02-042-5
5 x 200 ug
345 USD

Product
 
Information
Code:02-044

Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA (1). It plays important roles in DNA replication and recombination (2). Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa same as that of the natural protein.
Applications:
Stabilizes single-stranded DNA in DNA replication repair and recombination
Storage conditions:
50mM Tris-HCl (pH 8.0) 200mM NaCl 0.1mM dithiothreitol 0.5mM EDTA 50% glycerol
Store at -20℃
Activity:
Single-stranded DNA binding activity was confirmed (Fig.2).
Concentration:
1.0 mg/ml
Quality Assurance:
Greater than 95% of protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
 
 
Code
Quantity
                  Price
 
02-044
100 ug
200 USD

Product
 
Information
Code:02-048

Shipping and Storage: Ship at 4℃ and store at -20℃

Product: Recombinant Thermus aquaticus RecA protein expressed in E.
coli. Full-size, functional and no Tag-peptide attached.

Applications:
1) Useful for studying homologous recombination
2) Increase the specificity and yield of multiplex PCR (of cDNA or genomic DNA) by promoting homologous annealing of primers to target DNA (2)
3) Visualization of DNA with electoron microscopy due to nucleofilament formation.

Activity: The activity of single-stranded DNA-dependent ATPase was confirmed.

Form: 1 mg/ml in 50mM Tris-HCl (pH 8.0), 200mM NaCl, 1mM EDTA, 50% glycerol

Purity: More than 90% as judged from SDS-PAGE. No end- and exo-nuclease activity
 
 
Code
Quantity
                  Price
 
02-048
100 ug
150 USD

Product
 
Information
Code:02-050、02-050-5

Bacteriophage T4 derived DNA ligasecatalyzes the formation of phosphodiester bonds between 3’-OH termini and 5’-P termini in duplex DNA or RNA (1). This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA RNA or DNA/RNA hybrids。
  T4 DNA ligase was expressed in E.coli in large quantities and highly purified. MW is 55.3 kDa. 
Applications:
1) Insertion of DNA fragment into a vector
2) Linker (or Adaptor) ligation with DNA fragment
Storage conditions:
10mM Tris-HCl (pH 7.6) 50mM KCl 0.1mM EDTA 1mM dithiothreitol 50% glycerol
Store at -20℃
Concentration:
400 U/l where one unit is the amount of enzyme that ligates more than 90% of 6 g of DNA-HindIII fragments in a 20l mixture in 30 minutes at 16℃.
Quality Assurance:
Greater than 95% protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
 
 
Code
Quantity
                  Price
 
02-050
20000 U
35 USD
02-050-5
5x 20000 U
130 USD

Product
 
Information
code:02-060、02-060-5

Shipping and Storage: Ship at 4℃ or -20℃ and store at -20℃

Product: Recombinant full-size functional E.coli RNase H (=RNaseHI)
over-expressed in E. coli and highly purified. MW is 17.6 kDa.

Applications
1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the
second strand of cDNA
2) Removal of poly (A) tails from mRNA after hybridization with oligo
(dT)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA

Form: 50 units/ul in 20mM Tris-HCl (pH 7.5), 100mM KCl, 1mM DTT, 50%
Glycerol
Specific Activity: ~100,000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme
that hydrolyzes 1 nmol of the
RNA in 3H-labeled M13 DNA/RNA hybrid to acid-soluble
ribonucleotides in 20 min at 37℃.

Quality Assurance: Greater than 95% protein determined by SDS-PAGE
(CBB staining)Endo- and exo-DNase activities and RNase activity were
not detected with 100 U/ml RNaseH in 50 ul reaction at 37℃.

Reagents Supplied with Enzyme: RNaseH Reaction Buffer (10 X): 100 mM
Tris-HCl (pH 8.0), 100 mM MgCl2, 500 mM NaCl,10 mM DTT, 500 ug/ml
BSA (Bovine Serum Albumin)

 
 
Code
Quantity
                  Price
 
02-060
1000 U
110 USD
02-060-5
5 x 1000 U
330 USD

Product
 
Information
code:10-105

Mammalian DNA polymerase κ a member of the UmuC/DinB nucleotidyl transferase superfamily has been implicated in spontaneous mutagenesis (1). Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10-3 and 2 x 10-3 respectively. These error rates are high when compared to those of most other DNA polymerases (2). DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions (3).
This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa) and highly purified by several steps of chromatography (2). The product is catalytically active and its molecular weight is 65 kD (Fig 1). Activity of this product has been confirmed by a user researcher even if it was diluted 8000-fold.
Application
1) Functional studies on translesion DNA synthesis by DNA polymerase kappa
2) Antigen for western blotting and ELISA by anti-Pol kapp
 
 
Code
Quantity
                  Price
 
10-105
50 ug
490 USD

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