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Information
code:02-717

This cDNA library (plasmid DNA) is constructed from rat embryonic fibroblast-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express rat genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)

Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones: 62 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃
 
 
Code
Quantity
                  Price
 
02-717
500 ng
330 USD

Product
 
Information
code:02-719

This cDNA library (plasmid DNA) is constructed from poly(A)+ RNA of rat NRK (normal rat kidney) cells at the 0hr log phase. It is constructed by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express rat genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones: 1.4 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃
 
 
Code
Quantity
                  Price
 
02-719
500 ng
330 USD

Product
 
Information
code:02-721

This cDNA library (plasmid DNA) is constructed from Human Umbilical Vein Endothelial Cell (HUVEC)-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express human genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
 
 
Code
Quantity
                  Price
 
02-721
500 ng
330 USD

Product
 
Information
code:02-723

This cDNA library (plasmid DNA) is constructed from HeLa cell-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express human genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
 
 
Code
Quantity
                  Price
 
02-723
500 ng
330 USD

Product
 
Information
code:02-725

This cDNA library (plasmid DNA) is constructed from human TIG-1 cell (fetal lung fibroblast primary culture)-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pBA3 vector used in this library has pUC ori which enables replication in E. coli and Ampr as a selection marker.

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)

Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones: 8.6 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃
 
 
Code
Quantity
                  Price
 
02-725
500 ng
Price inquiry

Product
 
Information
code:02-727

This cDNA library (plasmid DNA) is constructed from poly(A)+ RNA of human TIG-1 cells (fetal lung fibroblasts) at contact inhibition state. It is constructed by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express rat genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468
Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)

Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones:50 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃
 
 
Code
Quantity
                  Price
 
02-727
500 ng
330 USD

Product
 
Information
Code:02-729

This cDNA library (plasmid DNA) is constructed from human embryonic kidney 293T cell -derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express human genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
 
 
Code
Quantity
                  Price
 
02-729
500 ng
330 USD

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