Key words: Acrosome reaction Membrane fusion Protein trafficking IZUMO1 Sperm–egg fusion
Function: Essential sperm cell-surface protein required for fertilization by acting as a ligand for FOLR4/JUNO receptor on egg.
The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane fusogen
Molecular mass: 44885 Da with 307 amino acids (mouse). Post-translational modification; Processing of N-terminal signal peptide with 21 amino acids. N-Glycosylation and phosphorylation.
Expression; Testis (low level) and sperm (high level). Not expressed in other organs.
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100 dilution)
3. Immunofluorescence staining (1/250 dilution))
Immunogen: Mouse sperm