Key words: Acrosome reaction Membrane fusion Protein trafficking IZUMO1 Sperm–egg fusion
Function: Essential sperm cell-surface protein required for fertilization by acting as a ligand for FOLR4/JUNO receptor on egg. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane fusogen
Molecular mass: 44885 Da with 307 amino acids. Post-translational modification; Processing of N-terminal signal peptide with 21 amino acids. N-Glucosylation and phosphorylation.
Expression: Small amount in testis and abundant in sperm. No expression in other organs.
1. Western blotting (1/1000)
2. Immunofluorescence staining (1/100~1/300)
3. Inhibition of sperm fusion with egg
Immunogen: KLH-conjugated synthetic peptides corresponding to the following three regions of human IZUMO 1.