Backgrouind: Legionnaires disease (LD) was recognized in 1976 after an outbreak of pneumonia at an American Legion convention in Philadelphia. Soon after the etiologic agent was identified as a fastidious gram-negative bacillus and named Legionella pneumophila. Although several other species of the genus Legionella were subsequently identified L pneumophila is the most frequent cause of human legionellosis and a relatively common cause of community-acquired and nosocomial pneumonia in adults. In children L pneumophila is also an important although relatively uncommon cause of pneumonia.
1) Immunofluorescent and Immunochemical staining (1/10000 ~1/30000 dilution)
2) Immunohistochemistry (1/3000~1/10000)
3) ELISA (1/10000~1/30000 dilution)
4) Agglutination (1/2000~1/5000)
Immunogen: Formaldehide treated whole cells of Legionella pneumophila strain Philadelphia 1 (ATCC #33152). Immunized 7 times at two weeks intervals.
Form: Undiluted antiserum added with 0.09% sodium azide.
Reactivity: Reacts with Legionella pneumophila strains. Since the antiserum has not been adsorbed it may cross-reacts with related bacteria.