Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn1 is a proteasome-interacting protein which acts as a chaperon to assemble the base sub-complex of the 19S RP. Rpn1 is composed of 993 amino acid residues with calculated molecular masses of 109 kDa .
1) Western blotting (1000~2000 fold dilution)
Not tested for other applications
Product: Rabbit antiserum added with 0.1% sodium azide
Immunogen: Synthetic peptide corresponding to a C-terminal region of Rpn (849~860).
Reactivity: S. cerevisiae Rpn1/Nas1. Not tested with other species