Influenza virus nucleoprotein (NP)Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is
abundantly expressed during the course of infection. It is a
structural protein which encapsidates the negative strand viral RNA and is essential for RNA transcription replication and packaging. NP binds the PB1 and PB2 subunits of the viral RNA polymerase and the matrix protein M1 in addition to its binding to ssRNA. NP is also known to interact with variety of other macromolecules of both viral and cellular origins and these interactions have been shown to be essential for the viral lifecycle.
Applications
1) Western blotting (300~1000 fold dilution)
2) Immuno-precipitation (100 fold dilution)
3) Immunofluorescent staining (200 fold dilution)
4) Immunohistochemistry (200 fold dilution)
5) ELISA (assay dependent)

http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=65-110

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9.4 kb which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. HCV core protein (191 aa) is not only a component of nucleocapsid but also has multiple functions and is thought to be a pathogenic factor for hepatitis. It also participates in some cellular processes including transcriptional regulation and cellular transduction. HCV core antigen is used as diagnostic marker for HCV infection.

Applications
1. Western blotting 2. Immunohistochemistry 3. Immunofluorescence staining
4. ELISA 5. FACS
http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=65-054

HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). p24 is used as a marker antigen for observing the patient’s condition after treatment as it indicates the amount of virus in the blood.
The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus subtype B (2) and was highly purified by several steps of chromatography (3 4).
Using this antiserum in Western blotting the bands of 24 kD 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells (Fig. 1).
This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=65-021

Cholera toxin a main enterotoxin interacts with G proteins and increases cyclic AMP in the intestinal lining to open ion channels. As ions flow into the intestinal lumen (lining) body fluids (mostly water) flows out of the body due to osmosis leading to massive diarrhea as the fluid is expelled from the body. Cholera toxin is a complex consisting of one molecule of A subunit (27.2 kD) and 5 molecules of B subunits (11.6 kD). After secretion A subunit is proteolytically processed into A1 (22 kD) and A2 (5 kD) subunits which are held together by a disulfide bond. The toxin adsorbs to GM1 ganglioside on the surface of target cells by the B subunit and the A subunit is dissociated from the B subunit during penetration. The A subunit constitutively activates adenyl cyclase activity of α subunit of Gs (a kind of GTP-binding protein).
http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=64-007

Tubulin is the major constituent of microtubules. There are three members (α β γ) and two subtypes (δ ε) in the tubulin family. Of these members Gamma tubulin (451aa 51KDa) is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome suggesting that it is involved in the minus-end nucleation of microtubule assembly during cell cycle.
Applications: : : :
1.Western blotting (200~ 1000 fold dilution)
2. Immunofluorescent staining (assay dependent)

http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=69-001

Schizosaccharomyces pombe Sds23 protein functions to facilitate the progression in anaphase in mitotic cell cycle. For initiating anaphase in fission yeast PP1 (protein phosphatase 1) and 20S cyclosome/APC are required. Thesds23 gene is a multicopy suppressor for mutations in PP1 and the 20S cyclosome/APC implying that the gene dosage increase can relieve the requirement for PP1 and the cyclosome/APC for the onset of anaphase. The sds23 gene encodes a 408 aa product and appears to be conserved. The sds23 gene is not essential for cell viability but in the sds23 deletion mutant the progression of anaphase and cytokinesis are retarded and cell shape is aberrant. Therefore Sds23 protein is involved in progression in anaphase as well as in cytokinesis and cell shape control. Sds23 is neither physically bound to PP1 nor a subunit of the cyclosome. It possibly regulates the PP1 and 20S cyclosome/APC in an unknown fashion.
http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=63-143

Rad21 protein (628 aa 67.8 kDa) is a cleavable component of the cohesin complex involved in chromosome cohesion during cell cycle. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition this protein is cleaved by Cut1 and dissociates from
chromatin allowing sister chromatids to segregate. Also involved in the DNA double-strand-break (DSB) repair system Hyperphosphorylated during S and G2 phases.
Proteolytic cleavage of a fraction of hyperphosphorylated form at the onset of anaphase may be essential for the proper progression of anaphase and sister chromatid separation. Belongs to the rad21 family conserved to mammals.
Applications
1. Western blotting (1/1000~1/4000 dilution) (Ref.1 & 2)
Not tested for other applications
http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=63-139

Cnd2 protein is a regulatory subunit of the condensin complex which is required for conversion of interphase chromatin into mitotic condensed chromosomes in S. pombe. The condensin complex also plays a role during interphase in processes of DNA repair and recombination.

Applications
1.Western blotting (500 fold dilution) (Ref.123)
2.Immunofluorescent staining (Ref. 3)
Not tested for other applications
http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=63-105

RagA (313 aa 36.6 kDa) is the human homologue of Gtr1 identified in yeast and classified as the Ras-like small G protein family. In cytoplasm GTP-bound RagA usually forms a heterodimer with RagD
interacts with Nop132 to be transported to the nucleous. GTP of RagA is hydrolyzed to GDP by RCC guanine nucleotide exchange factor for RanGTPase bound to chromatin. RagA-RCC signal pathway has a crucial role in cell growth and differentiation. RagA is also well known to be involved in mTOR signaling via binding with raptor a component of mTORC1 complex in an amino acid sensitive manner.
Applications
1) Western blotting ( 1000~ 2000 folds dilution)
Not tested for other applications



http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=71-020

DDX 47 gene encodes a member of the DEAD box protein family. DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Applications
1) Western blotting (~1000 fold dilution)
2) Immunofluorescence staining (~500 fold dilution)
Other applications have not been tested.

http://www.bioacademia.co.jp/en/product_list.php?srch_keyword=70-455